AMP-activated protein kinase induces p53 by phosphorylating MDMX and inhibiting its activity

Guifen He, Yi Wei Zhang, Jun Ho Lee, Shelya X. Zeng, Yunyuan V. Wang, Zhijun Luo, X. Dong, Benoit Viollet, Geoffrey M. Wahl, Hua Lu

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

AMP-activated protein kinase (AMPK) has been shown to activate p53 in response to metabolic stress. However, the underlying mechanisms remain unclear. Here we show that metabolic stresses induce AMPK-mediated phosphorylation of human MDMX on Ser342 in vitro and in cells, leading to enhanced association between MDMX and 14-3-3. This markedly inhibits p53 ubiquitylation and significantly stabilizes and activates p53. By striking contrast, no phosphorylation of MDM2 by AMPK was noted. AMPK-mediated MDMX phosphorylation, MDMX-14-3-3 binding, and p53 activation were drastically reduced in mouse embryo fibroblasts harboring endogenous MDMX with S341A (mouse homologue of human serine 342), S367A, and S402A (mouse homologue of human serine 403) mutations. Moreover, deficiency of AMPK prevented MDMX-14-3-3 interaction and p53 activation. The activation of p53 through AMPK-mediated MDMX phosphorylation and inactivation was further confirmed by using cell and animal model systems with two AMPK activators, metformin and salicylate (the active form of aspirin). Together, the results unveil a mechanism by which metabolic stresses activate AMPK, which, in turn, phosphorylates and inactivates MDMX, resulting in p53 stabilization and activation.

Original languageEnglish
Pages (from-to)148-157
Number of pages10
JournalMolecular and Cellular Biology
Volume34
Issue number2
DOIs
StatePublished - Jan 2014

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AMP-Activated Protein Kinases
Physiological Stress
Phosphorylation
Serine
Salicylates
Metformin
Ubiquitination
Aspirin
Embryonic Structures
Animal Models
Fibroblasts
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

He, G., Zhang, Y. W., Lee, J. H., Zeng, S. X., Wang, Y. V., Luo, Z., ... Lu, H. (2014). AMP-activated protein kinase induces p53 by phosphorylating MDMX and inhibiting its activity. Molecular and Cellular Biology, 34(2), 148-157. https://doi.org/10.1128/MCB.00670-13

AMP-activated protein kinase induces p53 by phosphorylating MDMX and inhibiting its activity. / He, Guifen; Zhang, Yi Wei; Lee, Jun Ho; Zeng, Shelya X.; Wang, Yunyuan V.; Luo, Zhijun; Dong, X.; Viollet, Benoit; Wahl, Geoffrey M.; Lu, Hua.

In: Molecular and Cellular Biology, Vol. 34, No. 2, 01.2014, p. 148-157.

Research output: Contribution to journalArticle

He, G, Zhang, YW, Lee, JH, Zeng, SX, Wang, YV, Luo, Z, Dong, X, Viollet, B, Wahl, GM & Lu, H 2014, 'AMP-activated protein kinase induces p53 by phosphorylating MDMX and inhibiting its activity', Molecular and Cellular Biology, vol. 34, no. 2, pp. 148-157. https://doi.org/10.1128/MCB.00670-13
He, Guifen ; Zhang, Yi Wei ; Lee, Jun Ho ; Zeng, Shelya X. ; Wang, Yunyuan V. ; Luo, Zhijun ; Dong, X. ; Viollet, Benoit ; Wahl, Geoffrey M. ; Lu, Hua. / AMP-activated protein kinase induces p53 by phosphorylating MDMX and inhibiting its activity. In: Molecular and Cellular Biology. 2014 ; Vol. 34, No. 2. pp. 148-157.
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