AMP-activated protein kinase is essential for survival in chronic hypoxia

Darrell R. Borger, L. Cristina Gavrilescu, Maria C. Bucur, Mircea Ivan, James A. DeCaprio

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This study was undertaken to interrogate cancer cell survival during long-term hypoxic stress. Two systems with relevance to carcinogenesis were employed: Fully transformed BJ cells and a renal carcinoma cell line (786-0). The dynamic of AMPK activity was consistent with a prosurvival role during chronic hypoxia. This was further supported by the effects of AMPK agonists and antagonists (AICAR and compound C). Expression of a dominant-negative AMPK alpha resulted in a decreased ATP level and significantly compromised survival in hypoxia. Dose-dependent prosurvival effects of rapamycin were consistent with mTOR inhibition being a critical downstream mediator of AMPK in persistent low oxygen.

Original languageEnglish (US)
Pages (from-to)230-234
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume370
Issue number2
DOIs
StatePublished - May 30 2008
Externally publishedYes

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AMP-Activated Protein Kinases
Cells
Sirolimus
Renal Cell Carcinoma
Cell Survival
Carcinogenesis
Adenosine Triphosphate
Oxygen
Cell Line
Hypoxia
Neoplasms

Keywords

  • AMPK
  • Apoptosis
  • Cancer
  • Hypoxia
  • mTOR
  • Rapamycin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

AMP-activated protein kinase is essential for survival in chronic hypoxia. / Borger, Darrell R.; Gavrilescu, L. Cristina; Bucur, Maria C.; Ivan, Mircea; DeCaprio, James A.

In: Biochemical and Biophysical Research Communications, Vol. 370, No. 2, 30.05.2008, p. 230-234.

Research output: Contribution to journalArticle

Borger, Darrell R. ; Gavrilescu, L. Cristina ; Bucur, Maria C. ; Ivan, Mircea ; DeCaprio, James A. / AMP-activated protein kinase is essential for survival in chronic hypoxia. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 370, No. 2. pp. 230-234.
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