AMPA receptor subunit expression in trigeminal neurons during postnatal development

Jack Turman, Andrew S. MacDonald, Kerry E.W. Pawl, Pablo Bringas, Scott H. Chandler

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Trigeminal motoneurons (Mo5) and mesencephalic trigeminal neurons (Me5) are important constituents of the neural circuitry responsible for jaw movements. Non-N-methyl-D-aspartate (NMDA)glutamate receptors are a critical component of the brainstem circuitry responsible for reflex and centrally activated jaw movements; however, little is known about the expression of these receptors in neonatal oral-motor circuitry. Receptor immunohistochemistry using affinity-purified polyclonal antibodies directed against GluR1, GluR2/3/4c, and GluR4, respectively, and a monoclonal antibody directed against the GluR2 subunit, were used in rats at postnatal day (P)1, P3, P5, P10, P19-21, P32-35, and P60 to describe the expression of the alpha-amino-d-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor in Mo5 and Me5 neurons. In Mo5, immunoreactivity was noted for all antibodies throughout the time frame sampled. Neurons in caudal portions of Me5 displayed immunoreactivity to each antibody except at P60 when GluR2 immunoreactivity was absent. Neurons located in rostral Me5 displayed GluR2/3/4c and GluR4 immunoreactivity throughout the time frame, GluR1 immunoreactivity emerged at P3 and a transient expression of GluR2 expression was observed between P10 and P32-35. The lack of labeling of some neurons in both regions, coupled with differences in temporal expression, suggests that there are differences in the AMPA receptor phenotype within and between Mo5 and Me5 during postnatal development. Differences in AMPA subunit composition suggest a complex role for AMPA-mediated glutamatergic neurotransmission in brainstem circuits controlling jaw movements. (C) 2000 WiLey-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)109-123
Number of pages15
JournalJournal of Comparative Neurology
Volume427
Issue number1
DOIs
StatePublished - Oct 16 2000
Externally publishedYes

Fingerprint

AMPA Receptors
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Neurons
Jaw
Brain Stem
Antibodies
D-Aspartic Acid
Glutamate Receptors
Motor Neurons
Synaptic Transmission
Reflex
Immunohistochemistry
Monoclonal Antibodies
Phenotype

Keywords

  • Glutamate
  • Immunohlstochemistry
  • Jaw
  • Suckling

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

AMPA receptor subunit expression in trigeminal neurons during postnatal development. / Turman, Jack; MacDonald, Andrew S.; Pawl, Kerry E.W.; Bringas, Pablo; Chandler, Scott H.

In: Journal of Comparative Neurology, Vol. 427, No. 1, 16.10.2000, p. 109-123.

Research output: Contribution to journalArticle

Turman, Jack ; MacDonald, Andrew S. ; Pawl, Kerry E.W. ; Bringas, Pablo ; Chandler, Scott H. / AMPA receptor subunit expression in trigeminal neurons during postnatal development. In: Journal of Comparative Neurology. 2000 ; Vol. 427, No. 1. pp. 109-123.
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