Ampicillin/sulbactam treatment modulates nmda receptor nr2b subunit and attenuates neuroinflammation and alcohol intake in male high alcohol drinking rats

Fawaz Alasmari, Hasan Alhaddad, Woonyen Wong, Richard L. Bell, Youssef Sari

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Exposure to ethanol commonly manifests neuroinflammation. Beta (β)-lactam antibiotics attenuate ethanol drinking through upregulation of astroglial glutamate transporters, especially glutamate transporter-1 (GLT-1), in the mesocorticolimbic brain regions, including the nucleus accumbens (Acb). However, the effect of β-lactam antibiotics on neuroinflammation in animals chronically exposed to ethanol has not been fully investigated. In this study, we evaluated the effects of ampicillin/sulbactam (AMP/SUL, 100 and 200 mg/kg, i.p.) on ethanol consumption in high alcohol drinking (HAD1) rats. Additionally, we investigated the effects of AMP/SUL on GLT-1 and N-methyl-D-aspartate (NMDA) receptor subtypes (NR2A and NR2B) in the Acb core (AcbCo) and Acb shell (AcbSh). We found that AMP/SUL at both doses attenuated ethanol consumption and restored ethanol-decreased GLT-1 and NR2B expression in the AcbSh and AcbCo, respectively. Moreover, AMP/SUL (200 mg/kg, i.p.) reduced ethanol-increased high mobility group box 1 (HMGB1) and receptor for advanced glycation end-products (RAGE) expression in the AcbSh. Moreover, both doses of AMP/SUL attenuated ethanol-elevated tumor necrosis factor-alpha (TNF-α) in the AcbSh. Our results suggest that AMP/SUL attenuates ethanol drinking and modulates NMDA receptor NR2B subunits and HMGB1-associated pathways.

Original languageEnglish (US)
Article number1030
Pages (from-to)1-17
Number of pages17
JournalBiomolecules
Volume10
Issue number7
DOIs
StatePublished - Jul 2020

Keywords

  • AMP/SUL
  • Ethanol
  • GLT-1
  • NMDA
  • Neuroinflammation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Ampicillin/sulbactam treatment modulates nmda receptor nr2b subunit and attenuates neuroinflammation and alcohol intake in male high alcohol drinking rats'. Together they form a unique fingerprint.

Cite this