Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green turtles with fibropapilloma: Brief Report

Andy Yu, Y. Lu, V. R. Nerurkar, R. Yanagihara

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A 1,632-bp fragment, flanking the original 483-bp region of the DNA polymerase gene of a novel herpesvirus found in tissues of green turtles (Chelonia mydas) with fibropapilloma, was amplified from the circularized EcoRI-cut DNA extracted from tumor tissues by inverse PCR. The resultant 2,019-bp partial sequence of the DNA polymerase gene of the newfound herpesvirus, including the original 483-bp region, showed a high degree of homology at both the nucleotide and amino acid levels with that of other human and animal herpesviruses. Phylogenetic analysis confirmed that this novel herpesvirus belonged to the Alphaherpesvirinae subfamily.

Original languageEnglish (US)
Pages (from-to)2669-2676
Number of pages8
JournalArchives of Virology
Volume145
Issue number12
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Turtles
Herpesviridae
DNA
DNA-Directed DNA Polymerase
Alphaherpesvirinae
Genes
Nucleotides
Amino Acids
Polymerase Chain Reaction
Neoplasms

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green turtles with fibropapilloma : Brief Report. / Yu, Andy; Lu, Y.; Nerurkar, V. R.; Yanagihara, R.

In: Archives of Virology, Vol. 145, No. 12, 2000, p. 2669-2676.

Research output: Contribution to journalArticle

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