Amplified increase in signal transduction activity in cancer cells

Hongyuan Yang, Fei Shen, George Weber

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Aim. To elucidate the behavior of 1 phosphatidylinositol 4,5 bisphosphate (PIP2) 5 phosphatase (PIP2 5-Pase, EC 3.1.3.36) and 1 phosphatidylinositol 4 phosphate (PIP) 4-phosphatase (PIP 4-Pase) in cancer cells, the steady state activities of PIP2 5-Pase and PIP 4-Pase were determined in the particulate fractions of rat liver and in a spectrum of rat hepatomas of different growth rates and malignancy. Methods. A standard method was developed using exogenous PIP2 or PIP as substrates. Results and Discussion. One half of the maximum activities was reached at about 0.3 mM of the substrates and at 0.1 to 0.2 mM magnesium chloride. The optimum concentrations of Triton X-100 and cetyltrimethyl ammonium bromide were 0.25% (w/v) and 1 mM, respectively. PIP2 5-Pase and PIP 4-Pase activities were linear with time for 20 min and proportional with protein concentrations up to 22 μg per 50 μl reaction mixture. In rat liver the steady state activities of PIP2 5-Pase and PIP 4-Pase were 1849 to 1881 and 1394 to 1670 nmol/h/mg protein. In three rat hepatomas the enzyme activities decreased to 50-51% and 33-42%, respectively. These results and our earlier data showing increased I phosphatidylinositol 4 kinase (EC 2.7.1.67), PIP 5-kinase (EC 2.7.1.68) and phospholipase C, PIP2 phosphodiesterase (EC 3.1.4.11) activities provide evidence of a transformation linked amplified increased capacity in signal transduction activity in cancer cells.

Original languageEnglish (US)
Pages (from-to)4983-4988
Number of pages6
JournalAnticancer Research
Volume19
Issue number6 B
StatePublished - Nov 1 1999

Keywords

  • 3924A
  • 5123 t.c.
  • Liver
  • PI kinase
  • PIP 4-Pase
  • PIP kinase
  • PIP 5-Pase
  • PLC
  • Rat hepatomas 9618A
  • Signal transduction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Amplified increase in signal transduction activity in cancer cells'. Together they form a unique fingerprint.

  • Cite this

    Yang, H., Shen, F., & Weber, G. (1999). Amplified increase in signal transduction activity in cancer cells. Anticancer Research, 19(6 B), 4983-4988.