An assessment of the interaction between human neutrophils, hydroxyapatite and dichloromethylene bisphosphonate in vitro: An electron microscopic and chemiluminescence study

P. M. Hyvonen, Michael Kowolik

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The potentially destructive effects of neutrophils, following particulate stimulus such as by hydroxyapatite (HA) crystals, may be pertinent to the progression of some inflammatory diseases, including rheumatoid arthritis. This study was designed to examine the interaction between pure hydroxyapatite particles and human neutrophil granulocytes by transmission electron microscopy and chemiluminescence (CL). The influence of dichloromethylene bisphosphonate (clodronate) in this model was investigated. Neutrophils convincingly phagocytosed HA particles within 15 min and a high CL response was elicited by the zymosan stimulated CL reaction. Clodronate alone appeared to have little effect on the cell morphology or CL. When HA was combined with clodronate, phagocytosis was more rapid, and the zymosan-stimulated CL was 50% of that of the HA group. The results demonstrate neutrophil phagocytosis of HA particles and subsequent cellular activation, which may play a role in the pathogenesis of tissue damage and suggest that the anti-inflammatory role of clodronate should be further investigated.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalJournal of Clinical and Laboratory Immunology
Volume35
Issue number2
StatePublished - 1991
Externally publishedYes

Fingerprint

Diphosphonates
Durapatite
Luminescence
Clodronic Acid
Neutrophils
Electrons
Phagocytosis
Zymosan
Transmission Electron Microscopy
Granulocytes
In Vitro Techniques
Rheumatoid Arthritis
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Immunology

Cite this

@article{1c8447962cf2475496cdea873437f944,
title = "An assessment of the interaction between human neutrophils, hydroxyapatite and dichloromethylene bisphosphonate in vitro: An electron microscopic and chemiluminescence study",
abstract = "The potentially destructive effects of neutrophils, following particulate stimulus such as by hydroxyapatite (HA) crystals, may be pertinent to the progression of some inflammatory diseases, including rheumatoid arthritis. This study was designed to examine the interaction between pure hydroxyapatite particles and human neutrophil granulocytes by transmission electron microscopy and chemiluminescence (CL). The influence of dichloromethylene bisphosphonate (clodronate) in this model was investigated. Neutrophils convincingly phagocytosed HA particles within 15 min and a high CL response was elicited by the zymosan stimulated CL reaction. Clodronate alone appeared to have little effect on the cell morphology or CL. When HA was combined with clodronate, phagocytosis was more rapid, and the zymosan-stimulated CL was 50{\%} of that of the HA group. The results demonstrate neutrophil phagocytosis of HA particles and subsequent cellular activation, which may play a role in the pathogenesis of tissue damage and suggest that the anti-inflammatory role of clodronate should be further investigated.",
author = "Hyvonen, {P. M.} and Michael Kowolik",
year = "1991",
language = "English (US)",
volume = "35",
pages = "47--55",
journal = "Journal of Clinical and Laboratory Immunology",
issn = "0141-2760",
publisher = "Teviot Scientific Publications Ltd.",
number = "2",

}

TY - JOUR

T1 - An assessment of the interaction between human neutrophils, hydroxyapatite and dichloromethylene bisphosphonate in vitro

T2 - An electron microscopic and chemiluminescence study

AU - Hyvonen, P. M.

AU - Kowolik, Michael

PY - 1991

Y1 - 1991

N2 - The potentially destructive effects of neutrophils, following particulate stimulus such as by hydroxyapatite (HA) crystals, may be pertinent to the progression of some inflammatory diseases, including rheumatoid arthritis. This study was designed to examine the interaction between pure hydroxyapatite particles and human neutrophil granulocytes by transmission electron microscopy and chemiluminescence (CL). The influence of dichloromethylene bisphosphonate (clodronate) in this model was investigated. Neutrophils convincingly phagocytosed HA particles within 15 min and a high CL response was elicited by the zymosan stimulated CL reaction. Clodronate alone appeared to have little effect on the cell morphology or CL. When HA was combined with clodronate, phagocytosis was more rapid, and the zymosan-stimulated CL was 50% of that of the HA group. The results demonstrate neutrophil phagocytosis of HA particles and subsequent cellular activation, which may play a role in the pathogenesis of tissue damage and suggest that the anti-inflammatory role of clodronate should be further investigated.

AB - The potentially destructive effects of neutrophils, following particulate stimulus such as by hydroxyapatite (HA) crystals, may be pertinent to the progression of some inflammatory diseases, including rheumatoid arthritis. This study was designed to examine the interaction between pure hydroxyapatite particles and human neutrophil granulocytes by transmission electron microscopy and chemiluminescence (CL). The influence of dichloromethylene bisphosphonate (clodronate) in this model was investigated. Neutrophils convincingly phagocytosed HA particles within 15 min and a high CL response was elicited by the zymosan stimulated CL reaction. Clodronate alone appeared to have little effect on the cell morphology or CL. When HA was combined with clodronate, phagocytosis was more rapid, and the zymosan-stimulated CL was 50% of that of the HA group. The results demonstrate neutrophil phagocytosis of HA particles and subsequent cellular activation, which may play a role in the pathogenesis of tissue damage and suggest that the anti-inflammatory role of clodronate should be further investigated.

UR - http://www.scopus.com/inward/record.url?scp=0026404632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026404632&partnerID=8YFLogxK

M3 - Article

C2 - 1668758

AN - SCOPUS:0026404632

VL - 35

SP - 47

EP - 55

JO - Journal of Clinical and Laboratory Immunology

JF - Journal of Clinical and Laboratory Immunology

SN - 0141-2760

IS - 2

ER -