An automated SPE-based high-yield synthesis of [ 11C]acetate and [ 11C]palmitate: No liquid-liquid extraction, solvent evaporation or distillation required

Bruce H. Mock, Clive Brown-Proctor, Mark A. Green, Brandon Steele, Barbara E. Glick-Wilson, Qi Huang Zheng

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Introduction: An automated method is described for the rapid and high-yield synthesis of two of the most commonly used radioactive fatty acids: [ 11C]acetate and [ 11C]palmitate. Methods: Reaction of [ 11C]CO 2 with the respective Grignard reagents in diethyl ether solution proceeded for 2 min at 40°C. Quenching of the reaction and liberation of nonreacted [ 11C]CO 2 occurred upon addition of a fourfold molar excess of aqueous 0.1 M HCl (acetate) or nonaqueous HCl/Et 2O (palmitate). Labeled products were then purified by adsorption to an Alumina-N Sep-Pak Plus cartridge and eluted with either aqueous NaH 2PO 4 solution (acetate) or 100% ethanol (palmitate). Results: High-performance liquid chromatography analysis confirmed that the radiochemical purity of each product was >98%, and decay-corrected radiochemical yields averaged 33% for [ 11C]palmitate and 40% for [ 11C]acetate. Conclusion: The method requires no liquid-liquid extraction, solvent evaporation or distillation capabilities and can be readily adapted to existing radiosynthesis modules.

Original languageEnglish (US)
Pages (from-to)1135-1142
Number of pages8
JournalNuclear Medicine and Biology
Volume38
Issue number8
DOIs
StatePublished - Nov 1 2011

Keywords

  • [ C]Acetate
  • [ C]Palmitate
  • Automation
  • Grignard reagent
  • Positron emission tomography (PET)
  • Solid-phase extraction (SPE)

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

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