An ELISA for blood group specific exoglycosidases

Lois Hobbs, Roy Phillips, Daniel Smith

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) for studying erythrocyte A, B and H epitope specific exoglycosidases is described. Human blood type B erythrocyte membranes and Coffea canephora α-d-galactosidase were used as a model. Membrane coated microtiter wells incubated with exoglycosidase, probed with IgM monoclonal antibody, and then with anti-murine μ chain specific alkaline phosphatase conjugate. The assay is useful for studying exoglycosidase modification of the A, B and H epitopes on human erythrocyte membranes as well as in screening prokaryotic and eukaryotic extracts for blood group active enzymes. Furthermore, this technique has the advantage of simplicity, sensitivity, and objectivity of data interpretation.

Original languageEnglish (US)
Pages (from-to)261-266
Number of pages6
JournalJournal of Immunological Methods
Volume160
Issue number2
DOIs
StatePublished - Apr 2 1993
Externally publishedYes

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Glycoside Hydrolases
Blood Group Antigens
Enzyme-Linked Immunosorbent Assay
Erythrocyte Membrane
Epitopes
Coffea
Galactosidases
Alkaline Phosphatase
Immunoglobulin M
Erythrocytes
Monoclonal Antibodies
Membranes
Enzymes

Keywords

  • Blood group antigen
  • ELISA
  • Exoglycosidase

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

An ELISA for blood group specific exoglycosidases. / Hobbs, Lois; Phillips, Roy; Smith, Daniel.

In: Journal of Immunological Methods, Vol. 160, No. 2, 02.04.1993, p. 261-266.

Research output: Contribution to journalArticle

Hobbs, Lois ; Phillips, Roy ; Smith, Daniel. / An ELISA for blood group specific exoglycosidases. In: Journal of Immunological Methods. 1993 ; Vol. 160, No. 2. pp. 261-266.
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