An ELISA method for quantitation of Pneumocystis carinii in culture and lung.

M. M. Durkin, M. S. Bartlett, S. F. Queener, M. M. Shaw, C. H. Lee, J. W. Smith

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1 Scopus citations

Abstract

Numbers of Pneumocystis carinii in cultures or tissues traditionally have been determined by counting organisms on Giemsa-stained slides. For cultures, 10 microliters of culture supernatants have been sampled and counted on days 1, 3, 5 and 7. Infectivity scores of P. carinii-infected animal lung have been determined by three examiners scoring lung impression smears stained with Giemsa using a roughly logarithmic scale. Both counting procedures are tedious and time consuming. We have developed an enzyme-linked immunosorbent assay (ELISA) system which uses culture supernatants (in vitro) or homogenized animal lung (in vivo) as antigen, convalescent rat sera as primary antibody, and goat anti-rat alkaline phosphatase-conjugated immunoglobulin G as secondary antibody. The ELISA method shows good correlation with manual counts of Giemsa stains and allows a more rapid, more efficient method for quantitating P. carinii in both culture and infected lung.

Original languageEnglish (US)
Pages (from-to)208S-210S
JournalThe Journal of Protozoology
Volume38
Issue number6
StatePublished - 1991

ASJC Scopus subject areas

  • Parasitology

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    Durkin, M. M., Bartlett, M. S., Queener, S. F., Shaw, M. M., Lee, C. H., & Smith, J. W. (1991). An ELISA method for quantitation of Pneumocystis carinii in culture and lung. The Journal of Protozoology, 38(6), 208S-210S.