An ELISA method for quantitation of Pneumocystis carinii in culture and lung.

M. M. Durkin, M. S. Bartlett, Sherry Queener, M. M. Shaw, Chao-Hung Lee, J. W. Smith

Research output: Contribution to journalArticle

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Abstract

Numbers of Pneumocystis carinii in cultures or tissues traditionally have been determined by counting organisms on Giemsa-stained slides. For cultures, 10 microliters of culture supernatants have been sampled and counted on days 1, 3, 5 and 7. Infectivity scores of P. carinii-infected animal lung have been determined by three examiners scoring lung impression smears stained with Giemsa using a roughly logarithmic scale. Both counting procedures are tedious and time consuming. We have developed an enzyme-linked immunosorbent assay (ELISA) system which uses culture supernatants (in vitro) or homogenized animal lung (in vivo) as antigen, convalescent rat sera as primary antibody, and goat anti-rat alkaline phosphatase-conjugated immunoglobulin G as secondary antibody. The ELISA method shows good correlation with manual counts of Giemsa stains and allows a more rapid, more efficient method for quantitating P. carinii in both culture and infected lung.

Original languageEnglish
JournalJournal of Protozoology
Volume38
Issue number6
StatePublished - Nov 1991

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Pneumocystis carinii
Enzyme-Linked Immunosorbent Assay
Lung
Azure Stains
Goats
Alkaline Phosphatase
Anti-Idiotypic Antibodies
Immunoglobulin G
Antigens
Antibodies
Serum

ASJC Scopus subject areas

  • Parasitology

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An ELISA method for quantitation of Pneumocystis carinii in culture and lung. / Durkin, M. M.; Bartlett, M. S.; Queener, Sherry; Shaw, M. M.; Lee, Chao-Hung; Smith, J. W.

In: Journal of Protozoology, Vol. 38, No. 6, 11.1991.

Research output: Contribution to journalArticle

Durkin, M. M. ; Bartlett, M. S. ; Queener, Sherry ; Shaw, M. M. ; Lee, Chao-Hung ; Smith, J. W. / An ELISA method for quantitation of Pneumocystis carinii in culture and lung. In: Journal of Protozoology. 1991 ; Vol. 38, No. 6.
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