An enzyme-linked immunosorbent assay for enumeration of Pneumocystis carinii in vitro and in vivo

M. M. Durkin, M. S. Bartlett, Sherry Queener, M. M. Shaw, Chao-Hung Lee, J. W. Smith

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) to quantitate Pneumocystis carinii organisms from culture supernatant and rat lung has been developed. A polyclonal antibody specific to P. carinii was produced in Sprague-Dawley rats by allowing P. carinii-infected animals to recover from infection. This antibody reacted strongly to P. carinii proteins of 50 to 55 kDa and weakly to those of 33 and 116 kDa. The ELISA used this convalescent-phase antibody to quantitate the number of P. carinii organisms in lung homogenates of infected rats and supernatants from infected tissue cultures which were used to screen drugs for P. carinii. The results of the ELISA were compared with those of direct microscopic counting of organisms, and the two methods were highly correlated (r > 0.9). Thus, the ELISA can be used as an alternative method for the quantitation of P. carinii organisms, and it is superior to the conventional microscopic method because it is easier to perform and less labor-intensive.

Original languageEnglish
Pages (from-to)3258-3262
Number of pages5
JournalJournal of Clinical Microbiology
Volume30
Issue number12
StatePublished - 1992

Fingerprint

Pneumocystis carinii
Enzyme-Linked Immunosorbent Assay
Antibodies
Lung
In Vitro Techniques
Sprague Dawley Rats
Infection
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

An enzyme-linked immunosorbent assay for enumeration of Pneumocystis carinii in vitro and in vivo. / Durkin, M. M.; Bartlett, M. S.; Queener, Sherry; Shaw, M. M.; Lee, Chao-Hung; Smith, J. W.

In: Journal of Clinical Microbiology, Vol. 30, No. 12, 1992, p. 3258-3262.

Research output: Contribution to journalArticle

@article{ef87f19b1e064e9b94df36eba8e08845,
title = "An enzyme-linked immunosorbent assay for enumeration of Pneumocystis carinii in vitro and in vivo",
abstract = "An enzyme-linked immunosorbent assay (ELISA) to quantitate Pneumocystis carinii organisms from culture supernatant and rat lung has been developed. A polyclonal antibody specific to P. carinii was produced in Sprague-Dawley rats by allowing P. carinii-infected animals to recover from infection. This antibody reacted strongly to P. carinii proteins of 50 to 55 kDa and weakly to those of 33 and 116 kDa. The ELISA used this convalescent-phase antibody to quantitate the number of P. carinii organisms in lung homogenates of infected rats and supernatants from infected tissue cultures which were used to screen drugs for P. carinii. The results of the ELISA were compared with those of direct microscopic counting of organisms, and the two methods were highly correlated (r > 0.9). Thus, the ELISA can be used as an alternative method for the quantitation of P. carinii organisms, and it is superior to the conventional microscopic method because it is easier to perform and less labor-intensive.",
author = "Durkin, {M. M.} and Bartlett, {M. S.} and Sherry Queener and Shaw, {M. M.} and Chao-Hung Lee and Smith, {J. W.}",
year = "1992",
language = "English",
volume = "30",
pages = "3258--3262",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - An enzyme-linked immunosorbent assay for enumeration of Pneumocystis carinii in vitro and in vivo

AU - Durkin, M. M.

AU - Bartlett, M. S.

AU - Queener, Sherry

AU - Shaw, M. M.

AU - Lee, Chao-Hung

AU - Smith, J. W.

PY - 1992

Y1 - 1992

N2 - An enzyme-linked immunosorbent assay (ELISA) to quantitate Pneumocystis carinii organisms from culture supernatant and rat lung has been developed. A polyclonal antibody specific to P. carinii was produced in Sprague-Dawley rats by allowing P. carinii-infected animals to recover from infection. This antibody reacted strongly to P. carinii proteins of 50 to 55 kDa and weakly to those of 33 and 116 kDa. The ELISA used this convalescent-phase antibody to quantitate the number of P. carinii organisms in lung homogenates of infected rats and supernatants from infected tissue cultures which were used to screen drugs for P. carinii. The results of the ELISA were compared with those of direct microscopic counting of organisms, and the two methods were highly correlated (r > 0.9). Thus, the ELISA can be used as an alternative method for the quantitation of P. carinii organisms, and it is superior to the conventional microscopic method because it is easier to perform and less labor-intensive.

AB - An enzyme-linked immunosorbent assay (ELISA) to quantitate Pneumocystis carinii organisms from culture supernatant and rat lung has been developed. A polyclonal antibody specific to P. carinii was produced in Sprague-Dawley rats by allowing P. carinii-infected animals to recover from infection. This antibody reacted strongly to P. carinii proteins of 50 to 55 kDa and weakly to those of 33 and 116 kDa. The ELISA used this convalescent-phase antibody to quantitate the number of P. carinii organisms in lung homogenates of infected rats and supernatants from infected tissue cultures which were used to screen drugs for P. carinii. The results of the ELISA were compared with those of direct microscopic counting of organisms, and the two methods were highly correlated (r > 0.9). Thus, the ELISA can be used as an alternative method for the quantitation of P. carinii organisms, and it is superior to the conventional microscopic method because it is easier to perform and less labor-intensive.

UR - http://www.scopus.com/inward/record.url?scp=0026439154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026439154&partnerID=8YFLogxK

M3 - Article

VL - 30

SP - 3258

EP - 3262

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 12

ER -