An essential domain within Cdc34p is required for binding to a complex containing Cdc4p and Cdc53p in Saccharomyces cerevisiae

Neal Mathias, C. Nic Steussy, Mark Goebl

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The CDC34 gene of the yeast Saccharomyces cerevisiae encodes a ubiquitin-conjugating protein that transfers ubiquitin onto substrates to signal rapid degradation via the proteasome. Cdc34p has been implicated in signaling the destruction of a variety of substrates including the cyclin- dependent kinase inhibitor, Sic1p, which must be degraded for cells to enter S-phase. Mutants lacking CDC34 activity fail to degrade Sic1p and fail to enter S-phase, a phenotype that is also shared with cells lacking CDC4 and CDC53 activity. Here we demonstrate that Cdc4p, Cdc34p, and Cdc53p interact in vivo. We have mapped a Cdc4p/Cdc53p-binding region on Cdc34p; this region is essential for S-phase entry and thus the association of these three proteins is required for Sic1p degradation. All three proteins migrate in gel filtration to sizes that greatly exceed their actual size suggesting that they form stable associations with other proteins and we observe Cdc4p, Cdc34p, and Cdc53p fractionating into overlapping families of high molecular weight complexes. Finally, we demonstrate that Cdc4p, Cdc34p, and Cdc53p are stable throughout the cell cycle and that Cdc34p permanently resides as part of a complex throughout the cell cycle. This suggests that all Cdc34p substrates are ubiquitinated by a similar high molecular weight complex.

Original languageEnglish
Pages (from-to)4040-4045
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number7
DOIs
StatePublished - Feb 13 1998

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Yeast
Saccharomyces cerevisiae
S Phase
Ubiquitin
Cell Cycle
Proteins
Substrates
Molecular Weight
Molecular weight
Cells
Degradation
Cyclin-Dependent Kinases
Proteasome Endopeptidase Complex
Gel Chromatography
Genes
Yeasts
Gels
Association reactions
Phenotype

ASJC Scopus subject areas

  • Biochemistry

Cite this

An essential domain within Cdc34p is required for binding to a complex containing Cdc4p and Cdc53p in Saccharomyces cerevisiae. / Mathias, Neal; Steussy, C. Nic; Goebl, Mark.

In: Journal of Biological Chemistry, Vol. 273, No. 7, 13.02.1998, p. 4040-4045.

Research output: Contribution to journalArticle

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