An evolutionary conserved target cell antigen along with MHC class I molecules influences susceptibility to murine NK cell lysis

Reuben Kapur, Donald L. Evans, David T. Harris

Research output: Contribution to journalArticle

Abstract

We have previously characterized a novel monoclonal antibody (mAb), termed 18C2, which binds to and inhibits the lysis of target cells by human natural killer (NK) cells. We now show that the anti-target cell mAb 18C2 also recognizes a similar structure on the murine NK sensitive target cell YAC-1, as well as on NK resistant target cells P815 and EL-4, as observed by flow cytometry. Functional studies demonstrated that the mAb 18C2 inhibited the lysis of both NK sensitive YAC-1 target cells, as well as NK resistant target cell lines P815 and EL-4 by freshly-isolated nylon wool nonadherent (NWNA) NK cells, 5-day lymphokine activated killer (LAK) cells and adherent lymphokine activated killer (ALAK) cells. The inhibitory activity of the mAb 18C2 occurred at the target cell level only. Single cell conjugate assays as demonstrated that the structure recognized by the mAb 18C2 was involved in recognition between NK cells and NK target cells, as the mAb inhibited conjugate formation between a variety of effector cells and various target cell lines tested. Further, the role of major bistocompatibility complex (MHC) class I antigens in NK cell cytotoxicity was examined. We observed that target cells expressing low levels of MHC class I antigens in association with the novel target cell antigen were more sensitive to NK cell lysis, as compared to cells that co-express higher levels of MHC class I antigen and the target cell antigen. Further, the presence of this antigen across different species suggests this target cell antigen/structure to be highly evolutionarily conserved.

Original languageEnglish (US)
Pages (from-to)347-355
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume19
Issue number4
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Keywords

  • Evolutionarily conserved
  • Lysis
  • NK cells
  • Recognition
  • Target antigen

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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