An Experimental Collagen-Impregnated Dacron Graft: Potential for Endothelial Seeding

Michael C. Dalsing, Melissa Kevorkian, Beth Raper, Craig Nixon, Stephen G. Lalka, Dolores F. Cikrit, Joseph L. Unthank, Malcolm B. Herring

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

This study evaluates the potential for endothelial seeding of a collagen-impregnated Dacron graft with or without surface modifiers (fibronectin, heparin) to attach and retain these cells during flow. Human umbilical endothelial cells were harvested, cultured, labeled with Indium111-oxine and seeded onto 30 mm × 4 mm diameter grafts. Six graft surfaces were studied: 1) a collagen-impregnated Dacron graft, HemashieldR (C); 2) C + fibronectin (C + F); 3) C + heparin (C + H); 4) C + F + H; 5) HytrelR + F (Hyt + F); and 6) Hyt + F + H. Radioactive loss determined the percentage attachment and then percentage retention of labeled inoculum after a one-hour in vitro perfusion. Scanning electron and light microscopy demonstrated the endothelium on the graft surface following perfusion. Fibronectin-coated grafts had a significantly higher percentage attachment than those without fibronectin (ANOVA, P < 0.05). However, the percentage retention following perfusion was similar for all Dacron grafts and statistically inferior to the HytrelR grafts studied (ANOVA, P < 0.05). SEM evaluation of the C + F + H graft surface was qualitatively the most impressive Dacron surface for seeding, yet was inferior to the HytrelR graft. We conclude that fibronectin benefits the initial attachment of endothelium to collagen-coated Dacron rivaling the HytrelR surface. Fibronectin does not improve percentage retention of the HemashieldR surface during perfusion, therefore, some of its initial benefit is lost.

Original languageEnglish (US)
Pages (from-to)127-133
Number of pages7
JournalAnnals of Vascular Surgery
Volume3
Issue number2
DOIs
StatePublished - Jan 1 1989

Keywords

  • Endothelial seeding
  • collagen-impregnated grafts
  • fibronectin
  • grafts

ASJC Scopus subject areas

  • Surgery
  • Cardiology and Cardiovascular Medicine

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