An extremely simple method for extraction of lysophospholipids and phospholipids from blood samples

Zhenwen Zhao, Yan Xu

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

Lipids, lysophospholipids and phospholipids in particular, have been shown to be biomarkers and potential therapeutic targets for human diseases. While many extraction and analytical methods have been developed for quantitative analyses of these molecules, most of them are laborious and time-consuming, with associated issues of poor reproducibility. This becomes one of the critical bottle-necks to move lipid markers to clinics. In the current work, we have developed an extremely simple method for lysophospholipids and phospholipids extraction from human plasma or serum samples, which only utilizes a single methanol (MeOH) solvent and involves a single step of centrifugation. This method has been subjected to strict validation by comparing it with classical lipid extraction methods. This simple method will be extremely useful for the lipidomic, diseases marker, and lipid biochemistry fields not only for its potential wide applications associated with its simplicity and reproducibility, but also for its impact in moving lipid markers into clinics through high-throughput processing.

Original languageEnglish (US)
Pages (from-to)652-659
Number of pages8
JournalJournal of Lipid Research
Volume51
Issue number3
DOIs
StatePublished - Mar 1 2010

Keywords

  • Cell pellet lipid
  • HPLC-ESI-MS/MS
  • MeOH method
  • Tissue lipid

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

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