An improved intra-oral enamel demineralization test model for the study of dental caries.

Domenick Zero, J. Fu, K. M. Anne, S. Cassata, S. M. McCormack, L. M. Gwinner

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The intra-oral enamel demineralization test (IEDT) was introduced by Brudevold et al. (1984). This caries model involves human subjects wearing palatal appliances each holding eight bovine enamel blocks covered by a bacterial cell layer prepared by the harvesting of cultures of Streptococcus mutants (test plaque). The original model used the iodide permeability test for assessment of the extent of demineralization of bovine enamel blocks resulting from acid production by the test plaque after dietary substrate challenge. The IEDT model has been expanded and improved by us in the following ways: (1) Based on encouraging findings from an in vitro study (Zero et al., 1990), the surface microhardness test has been adopted to measure the extent of demineralization occurring at three sites on the enamel blocks corresponding to an area over which the effective plaque thickness is 0.5, 1.5, and 2.5 mm; (2) intra-oral pH of the test plaque is measured by means of a Beetrode miniature pH electrode at baseline, then at five, 10, 15, 30, and 45 min after the start of a test; (3) plaque samples are collected at the end of a test and analyzed for organic acid content by means of HPLC; (4) the bacterial test challenge has been expanded to include different cariogenic bacteria which are grown under various growth conditions. The improved model has the capability of studying fundamental aspects of the caries process, namely, the relationships among dietary substrate challenge, plaque pH change, plaque organic acid profiles, microbial virulence properties, and enamel demineralization. Furthermore, the model has the potential for use in more applied research on caries-preventive agents such as fluoride.

Original languageEnglish (US)
Pages (from-to)871-878
Number of pages8
JournalJournal of Dental Research
Volume71 Spec No
StatePublished - Apr 1992
Externally publishedYes

Fingerprint

Dental Caries
Dental Enamel
Acids
Iodides
Streptococcus
Fluorides
Virulence
Permeability
Electrodes
High Pressure Liquid Chromatography
Bacteria
Growth
Research

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Zero, D., Fu, J., Anne, K. M., Cassata, S., McCormack, S. M., & Gwinner, L. M. (1992). An improved intra-oral enamel demineralization test model for the study of dental caries. Journal of Dental Research, 71 Spec No, 871-878.

An improved intra-oral enamel demineralization test model for the study of dental caries. / Zero, Domenick; Fu, J.; Anne, K. M.; Cassata, S.; McCormack, S. M.; Gwinner, L. M.

In: Journal of Dental Research, Vol. 71 Spec No, 04.1992, p. 871-878.

Research output: Contribution to journalArticle

Zero, D, Fu, J, Anne, KM, Cassata, S, McCormack, SM & Gwinner, LM 1992, 'An improved intra-oral enamel demineralization test model for the study of dental caries.', Journal of Dental Research, vol. 71 Spec No, pp. 871-878.
Zero D, Fu J, Anne KM, Cassata S, McCormack SM, Gwinner LM. An improved intra-oral enamel demineralization test model for the study of dental caries. Journal of Dental Research. 1992 Apr;71 Spec No:871-878.
Zero, Domenick ; Fu, J. ; Anne, K. M. ; Cassata, S. ; McCormack, S. M. ; Gwinner, L. M. / An improved intra-oral enamel demineralization test model for the study of dental caries. In: Journal of Dental Research. 1992 ; Vol. 71 Spec No. pp. 871-878.
@article{b0fe801ddce7435f81ea7123ddb32ddd,
title = "An improved intra-oral enamel demineralization test model for the study of dental caries.",
abstract = "The intra-oral enamel demineralization test (IEDT) was introduced by Brudevold et al. (1984). This caries model involves human subjects wearing palatal appliances each holding eight bovine enamel blocks covered by a bacterial cell layer prepared by the harvesting of cultures of Streptococcus mutants (test plaque). The original model used the iodide permeability test for assessment of the extent of demineralization of bovine enamel blocks resulting from acid production by the test plaque after dietary substrate challenge. The IEDT model has been expanded and improved by us in the following ways: (1) Based on encouraging findings from an in vitro study (Zero et al., 1990), the surface microhardness test has been adopted to measure the extent of demineralization occurring at three sites on the enamel blocks corresponding to an area over which the effective plaque thickness is 0.5, 1.5, and 2.5 mm; (2) intra-oral pH of the test plaque is measured by means of a Beetrode miniature pH electrode at baseline, then at five, 10, 15, 30, and 45 min after the start of a test; (3) plaque samples are collected at the end of a test and analyzed for organic acid content by means of HPLC; (4) the bacterial test challenge has been expanded to include different cariogenic bacteria which are grown under various growth conditions. The improved model has the capability of studying fundamental aspects of the caries process, namely, the relationships among dietary substrate challenge, plaque pH change, plaque organic acid profiles, microbial virulence properties, and enamel demineralization. Furthermore, the model has the potential for use in more applied research on caries-preventive agents such as fluoride.",
author = "Domenick Zero and J. Fu and Anne, {K. M.} and S. Cassata and McCormack, {S. M.} and Gwinner, {L. M.}",
year = "1992",
month = "4",
language = "English (US)",
volume = "71 Spec No",
pages = "871--878",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",

}

TY - JOUR

T1 - An improved intra-oral enamel demineralization test model for the study of dental caries.

AU - Zero, Domenick

AU - Fu, J.

AU - Anne, K. M.

AU - Cassata, S.

AU - McCormack, S. M.

AU - Gwinner, L. M.

PY - 1992/4

Y1 - 1992/4

N2 - The intra-oral enamel demineralization test (IEDT) was introduced by Brudevold et al. (1984). This caries model involves human subjects wearing palatal appliances each holding eight bovine enamel blocks covered by a bacterial cell layer prepared by the harvesting of cultures of Streptococcus mutants (test plaque). The original model used the iodide permeability test for assessment of the extent of demineralization of bovine enamel blocks resulting from acid production by the test plaque after dietary substrate challenge. The IEDT model has been expanded and improved by us in the following ways: (1) Based on encouraging findings from an in vitro study (Zero et al., 1990), the surface microhardness test has been adopted to measure the extent of demineralization occurring at three sites on the enamel blocks corresponding to an area over which the effective plaque thickness is 0.5, 1.5, and 2.5 mm; (2) intra-oral pH of the test plaque is measured by means of a Beetrode miniature pH electrode at baseline, then at five, 10, 15, 30, and 45 min after the start of a test; (3) plaque samples are collected at the end of a test and analyzed for organic acid content by means of HPLC; (4) the bacterial test challenge has been expanded to include different cariogenic bacteria which are grown under various growth conditions. The improved model has the capability of studying fundamental aspects of the caries process, namely, the relationships among dietary substrate challenge, plaque pH change, plaque organic acid profiles, microbial virulence properties, and enamel demineralization. Furthermore, the model has the potential for use in more applied research on caries-preventive agents such as fluoride.

AB - The intra-oral enamel demineralization test (IEDT) was introduced by Brudevold et al. (1984). This caries model involves human subjects wearing palatal appliances each holding eight bovine enamel blocks covered by a bacterial cell layer prepared by the harvesting of cultures of Streptococcus mutants (test plaque). The original model used the iodide permeability test for assessment of the extent of demineralization of bovine enamel blocks resulting from acid production by the test plaque after dietary substrate challenge. The IEDT model has been expanded and improved by us in the following ways: (1) Based on encouraging findings from an in vitro study (Zero et al., 1990), the surface microhardness test has been adopted to measure the extent of demineralization occurring at three sites on the enamel blocks corresponding to an area over which the effective plaque thickness is 0.5, 1.5, and 2.5 mm; (2) intra-oral pH of the test plaque is measured by means of a Beetrode miniature pH electrode at baseline, then at five, 10, 15, 30, and 45 min after the start of a test; (3) plaque samples are collected at the end of a test and analyzed for organic acid content by means of HPLC; (4) the bacterial test challenge has been expanded to include different cariogenic bacteria which are grown under various growth conditions. The improved model has the capability of studying fundamental aspects of the caries process, namely, the relationships among dietary substrate challenge, plaque pH change, plaque organic acid profiles, microbial virulence properties, and enamel demineralization. Furthermore, the model has the potential for use in more applied research on caries-preventive agents such as fluoride.

UR - http://www.scopus.com/inward/record.url?scp=0026845655&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026845655&partnerID=8YFLogxK

M3 - Article

VL - 71 Spec No

SP - 871

EP - 878

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

ER -