An improved total synthesis of [18F]FHPG starting from 1,3-dibenzyloxy-2-propanol and guanine has been developed. [18F]FHPG was prepared by nucleophilic substitution of the appropriate precursor with [18F]KF/ Kryptofix 2.2.2 followed by a quick deprotection reaction and purification with a simplified Silica Sep-Pak solid-phase extraction (SPE) method in 10-15% radiochemical yield, and 70 min synthesis time from end of bombardment (EOB).
- 9-[(3-[F]Fluoro-1-hydroxy-2-propoxy)methyl]guanine ([ F]FHPG)
- Ganciclovir (GCV)
- Gene expression
- Herpes simplex virus thymidine kinase (HSV-tk)
- Positron emission tomography (PET)
ASJC Scopus subject areas
- Organic Chemistry