An Improved Total Synthesis of PET HSV-tk Gene Reporter Probe 9-(4-[ 18F]Fluoro-3-hydroxymethylbutyl)guanine ([18F]FHBG)

Qi Huang Zheng, Ji Quan Wang, Xuan Liu, Xiangshu Fei, Bruce H. Mock, Barbara E. Glick-Wilson, Michael L. Sullivan, Sudhanshu P. Raikwar, Thomas A. Gardner, Chinghai Kao, Gary D. Hutchins

Research output: Contribution to journalArticle

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An improved total synthesis of [18F]FHBG starting from triethyl-1,1,2-ethanetricarboxylate and 2-amino-6-chloropurine is reported. [18F]FHBG was prepared by nucleophilic substitution of the appropriate precursor with [18F]KF/Kryptofix 2.2.2 followed by a quick deprotection reaction and purification with a simplified Silica Sep-Pak solid-phase extraction (SPE) method in 20-25% radiochemical yield.

Original languageEnglish (US)
Pages (from-to)689-704
Number of pages16
JournalSynthetic Communications
Issue number4
StatePublished - Mar 26 2004



  • 9-(4-[F]fluoro-3-hydroxymethylbutyl)guanine ([ F]FHBG)
  • Gene expression
  • Herpes simplex virus thymidine kinase (HSV-tk)
  • Penciclovir (PCV)
  • Positron emission tomography (PET)
  • Synthesis

ASJC Scopus subject areas

  • Organic Chemistry

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