An improved total synthesis of [18F]FHBG starting from triethyl-1,1,2-ethanetricarboxylate and 2-amino-6-chloropurine is reported. [18F]FHBG was prepared by nucleophilic substitution of the appropriate precursor with [18F]KF/Kryptofix 2.2.2 followed by a quick deprotection reaction and purification with a simplified Silica Sep-Pak solid-phase extraction (SPE) method in 20-25% radiochemical yield.
- 9-(4-[F]fluoro-3-hydroxymethylbutyl)guanine ([ F]FHBG)
- Gene expression
- Herpes simplex virus thymidine kinase (HSV-tk)
- Penciclovir (PCV)
- Positron emission tomography (PET)
ASJC Scopus subject areas
- Organic Chemistry