An in situ model for simultaneous assessment of inhibition of demineralization and enhancement of remineralization.

J. D. Featherstone, Domenick Zero

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

In situ models to assess the ability of oral care products or food components to enhance remineralization and/or inhibit demineralization of tooth enamel or roots must be very carefully designed to minimize the confounding effects of the many variables involved. Controlling these variables as closely as possible is essential if meaningful answers are to be obtained from the models. We have developed an in situ model which combines the experience of several groups. Detailed screening of subjects is essential. Selection criteria should include good general health, good dental health, mandibular partial denture, at least eight natural teeth, no active caries lesions, known fluoride history, normal salivary function, and no medications that affect salivary function. Each subject carries a sound enamel slab and an enamel slab with a pre-formed caries-like lesion (demineralized in vitro) in his/her denture on each side of the mouth for test periods of two or four weeks. The demineralization challenge is controlled by extra-oral immersion of the appliances in sucrose daily. Daily product exposure or daily food component exposure is used as desired. Compliance indicators and a diet diary are included. Whole saliva flow rate (unstimulated), plaque acidogenicity, and salivary fluoride are monitored during the test periods. At the end of the test period, the test slabs are assessed for mineral change, after being sectioned, by means of cross-sectional microhardness or microradiography. The mineral loss or gain (delta M, microns x vol%), compared with adjacent control sections retained in the lab, is calculated as change in delta Z (microns x vol%), namely, delta M = delta ZTEST - delta ZCONTROL.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)804-810
Number of pages7
JournalJournal of Dental Research
Volume71 Spec No
StatePublished - Apr 1992
Externally publishedYes

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Dental Enamel
Fluorides
Minerals
Tooth Demineralization
Tooth
Microradiography
Partial Denture
Food
Dentures
Health
Immersion
Saliva
Patient Selection
Sucrose
Mouth
History
Diet

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

An in situ model for simultaneous assessment of inhibition of demineralization and enhancement of remineralization. / Featherstone, J. D.; Zero, Domenick.

In: Journal of Dental Research, Vol. 71 Spec No, 04.1992, p. 804-810.

Research output: Contribution to journalArticle

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