An in vitro microbial model associated with sucrose to produce dentin caries lesions

Carolina Steiner-Oliveira, Lidiany Karla Azevedo Rodrigues, Iriana Carla Junqueira Zanin, Carolina Lima de Carvalho, Regianne Umeko Kamiya, Anderson Hara, Marinês Nobre-dos-Santos

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The complexity of the oral environment and ethical issues have prompted the development of an in vitro bacterial model to evaluate the effect of frequency of sucrose exposure on dentin caries formation, biofilm composition, and pH changes. In the experiment, dentin specimens (n=45) were randomly divided into four groups: control (C), negative control (0S), 3S (three sucrose baths), and 6S (six sucrose baths). The specimens then were inoculated with Streptococcus mutans and treated according to the protocol described below. Dentin demineralization and lesion depth were assessed by transverse microradiography. Extracellular polysaccharides that formed in the biofilm were analyzed and counts of microorganisms in the carious dentin were measured. After a 7-day period of growth, the biofilm pH was assessed before and after sucrose baths (n=5). The addition of sucrose led to dentin caries development regardless of the number of sucrose baths performed. The number of colony forming units (cfu) from the carious dentin did not differ among the treatment groups, though the extracellular polysaccharides from both 3S and 6S differed from 0S. The pH decreased immediately after the sucrose bath but increased again after 5 min. We demonstrate here that the in vitro microbial model for the study of dentin caries formation is reproducible and able to produce dentin caries, irrespective of the frequency of sucrose exposure.

Original languageEnglish (US)
Pages (from-to)414-421
Number of pages8
JournalCentral European Journal of Biology
Volume6
Issue number3
DOIs
StatePublished - Jun 2011

Fingerprint

Dentin
lesions (animal)
Sucrose
teeth
sucrose
Baths
Biofilms
biofilm
Dental Caries
Polysaccharides
polysaccharides
Microradiography
Streptococcus mutans
In Vitro Techniques
Ethics
Microorganisms
mouth
Stem Cells
microorganisms
Control Groups

Keywords

  • Artificial saliva
  • Biofilm pH
  • Microradiography
  • Streptococcus mutans

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Steiner-Oliveira, C., Rodrigues, L. K. A., Zanin, I. C. J., de Carvalho, C. L., Kamiya, R. U., Hara, A., & Nobre-dos-Santos, M. (2011). An in vitro microbial model associated with sucrose to produce dentin caries lesions. Central European Journal of Biology, 6(3), 414-421. https://doi.org/10.2478/s11535-011-0011-2

An in vitro microbial model associated with sucrose to produce dentin caries lesions. / Steiner-Oliveira, Carolina; Rodrigues, Lidiany Karla Azevedo; Zanin, Iriana Carla Junqueira; de Carvalho, Carolina Lima; Kamiya, Regianne Umeko; Hara, Anderson; Nobre-dos-Santos, Marinês.

In: Central European Journal of Biology, Vol. 6, No. 3, 06.2011, p. 414-421.

Research output: Contribution to journalArticle

Steiner-Oliveira, C, Rodrigues, LKA, Zanin, ICJ, de Carvalho, CL, Kamiya, RU, Hara, A & Nobre-dos-Santos, M 2011, 'An in vitro microbial model associated with sucrose to produce dentin caries lesions', Central European Journal of Biology, vol. 6, no. 3, pp. 414-421. https://doi.org/10.2478/s11535-011-0011-2
Steiner-Oliveira, Carolina ; Rodrigues, Lidiany Karla Azevedo ; Zanin, Iriana Carla Junqueira ; de Carvalho, Carolina Lima ; Kamiya, Regianne Umeko ; Hara, Anderson ; Nobre-dos-Santos, Marinês. / An in vitro microbial model associated with sucrose to produce dentin caries lesions. In: Central European Journal of Biology. 2011 ; Vol. 6, No. 3. pp. 414-421.
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