An in vitro Microbial Model for Studying Secondary Caries Formation

M. Fontana, A. J. Dunipace, Richard Gregory, T. W. Noblitt, Y. Li, K. K. Park, G. K. Stookey

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Secondary caries is a major reason for the replacement of restorations. Because it is hypothesized that the development of secondary caries is closely associated with pathogenic oral bacteria, an in vitro microbial model has been developed to produce secondary carious lesions. A mixture of overnight cultures of Streptococcus mutons and Lactobacillus casei in dextrose-free trypticase soy broth, supplemented with 5% sucrose (TSBS), at 37°C was used in this model as the inoculum for the experimental groups. Uninoculated control groups were incubated with medium only. Groups of human tooth specimes restored using composite, together with their respective controls, were exposed for 7 or 12 days to circulating cycles of TSBS (30 min each, 3 times per day) and a mineral wash solution (for a total of 22.5 h per day), at 37°C. The pH of the experimental groups dropped to 4.1-4.5 during the test periods. The pH of the control groups remained at 6.8-7.0. The inoculated bacteria remained viable throughout the study. No contamination of experimental or control samples occurred. Laser scanning confocal microscopy demonstrated the development of incipient sur-face and wall lesions in all the specimens of experimental groups in as few as 7 days. Reproducibility of the model was confirmed in a second investigation. Therefore, it was concluded that this model can be used for studying the microbial etiology and prevention of secondary caries.

Original languageEnglish
Pages (from-to)112-118
Number of pages7
JournalCaries Research
Volume30
Issue number2
StatePublished - Mar 1996

Fingerprint

Sucrose
Bacteria
Lactobacillus casei
Control Groups
Secondary Prevention
Streptococcus
Confocal Microscopy
Minerals
Tooth
Glucose
In Vitro Techniques
trypticase-soy broth

Keywords

  • In vitro microbial model
  • Lactobacillus casei
  • Secondary caries
  • Streptococcus mutans

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Fontana, M., Dunipace, A. J., Gregory, R., Noblitt, T. W., Li, Y., Park, K. K., & Stookey, G. K. (1996). An in vitro Microbial Model for Studying Secondary Caries Formation. Caries Research, 30(2), 112-118.

An in vitro Microbial Model for Studying Secondary Caries Formation. / Fontana, M.; Dunipace, A. J.; Gregory, Richard; Noblitt, T. W.; Li, Y.; Park, K. K.; Stookey, G. K.

In: Caries Research, Vol. 30, No. 2, 03.1996, p. 112-118.

Research output: Contribution to journalArticle

Fontana, M, Dunipace, AJ, Gregory, R, Noblitt, TW, Li, Y, Park, KK & Stookey, GK 1996, 'An in vitro Microbial Model for Studying Secondary Caries Formation', Caries Research, vol. 30, no. 2, pp. 112-118.
Fontana M, Dunipace AJ, Gregory R, Noblitt TW, Li Y, Park KK et al. An in vitro Microbial Model for Studying Secondary Caries Formation. Caries Research. 1996 Mar;30(2):112-118.
Fontana, M. ; Dunipace, A. J. ; Gregory, Richard ; Noblitt, T. W. ; Li, Y. ; Park, K. K. ; Stookey, G. K. / An in vitro Microbial Model for Studying Secondary Caries Formation. In: Caries Research. 1996 ; Vol. 30, No. 2. pp. 112-118.
@article{5b872bf17d0b4af7be4d5466fb3eaa00,
title = "An in vitro Microbial Model for Studying Secondary Caries Formation",
abstract = "Secondary caries is a major reason for the replacement of restorations. Because it is hypothesized that the development of secondary caries is closely associated with pathogenic oral bacteria, an in vitro microbial model has been developed to produce secondary carious lesions. A mixture of overnight cultures of Streptococcus mutons and Lactobacillus casei in dextrose-free trypticase soy broth, supplemented with 5{\%} sucrose (TSBS), at 37°C was used in this model as the inoculum for the experimental groups. Uninoculated control groups were incubated with medium only. Groups of human tooth specimes restored using composite, together with their respective controls, were exposed for 7 or 12 days to circulating cycles of TSBS (30 min each, 3 times per day) and a mineral wash solution (for a total of 22.5 h per day), at 37°C. The pH of the experimental groups dropped to 4.1-4.5 during the test periods. The pH of the control groups remained at 6.8-7.0. The inoculated bacteria remained viable throughout the study. No contamination of experimental or control samples occurred. Laser scanning confocal microscopy demonstrated the development of incipient sur-face and wall lesions in all the specimens of experimental groups in as few as 7 days. Reproducibility of the model was confirmed in a second investigation. Therefore, it was concluded that this model can be used for studying the microbial etiology and prevention of secondary caries.",
keywords = "In vitro microbial model, Lactobacillus casei, Secondary caries, Streptococcus mutans",
author = "M. Fontana and Dunipace, {A. J.} and Richard Gregory and Noblitt, {T. W.} and Y. Li and Park, {K. K.} and Stookey, {G. K.}",
year = "1996",
month = "3",
language = "English",
volume = "30",
pages = "112--118",
journal = "Caries Research",
issn = "0008-6568",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - An in vitro Microbial Model for Studying Secondary Caries Formation

AU - Fontana, M.

AU - Dunipace, A. J.

AU - Gregory, Richard

AU - Noblitt, T. W.

AU - Li, Y.

AU - Park, K. K.

AU - Stookey, G. K.

PY - 1996/3

Y1 - 1996/3

N2 - Secondary caries is a major reason for the replacement of restorations. Because it is hypothesized that the development of secondary caries is closely associated with pathogenic oral bacteria, an in vitro microbial model has been developed to produce secondary carious lesions. A mixture of overnight cultures of Streptococcus mutons and Lactobacillus casei in dextrose-free trypticase soy broth, supplemented with 5% sucrose (TSBS), at 37°C was used in this model as the inoculum for the experimental groups. Uninoculated control groups were incubated with medium only. Groups of human tooth specimes restored using composite, together with their respective controls, were exposed for 7 or 12 days to circulating cycles of TSBS (30 min each, 3 times per day) and a mineral wash solution (for a total of 22.5 h per day), at 37°C. The pH of the experimental groups dropped to 4.1-4.5 during the test periods. The pH of the control groups remained at 6.8-7.0. The inoculated bacteria remained viable throughout the study. No contamination of experimental or control samples occurred. Laser scanning confocal microscopy demonstrated the development of incipient sur-face and wall lesions in all the specimens of experimental groups in as few as 7 days. Reproducibility of the model was confirmed in a second investigation. Therefore, it was concluded that this model can be used for studying the microbial etiology and prevention of secondary caries.

AB - Secondary caries is a major reason for the replacement of restorations. Because it is hypothesized that the development of secondary caries is closely associated with pathogenic oral bacteria, an in vitro microbial model has been developed to produce secondary carious lesions. A mixture of overnight cultures of Streptococcus mutons and Lactobacillus casei in dextrose-free trypticase soy broth, supplemented with 5% sucrose (TSBS), at 37°C was used in this model as the inoculum for the experimental groups. Uninoculated control groups were incubated with medium only. Groups of human tooth specimes restored using composite, together with their respective controls, were exposed for 7 or 12 days to circulating cycles of TSBS (30 min each, 3 times per day) and a mineral wash solution (for a total of 22.5 h per day), at 37°C. The pH of the experimental groups dropped to 4.1-4.5 during the test periods. The pH of the control groups remained at 6.8-7.0. The inoculated bacteria remained viable throughout the study. No contamination of experimental or control samples occurred. Laser scanning confocal microscopy demonstrated the development of incipient sur-face and wall lesions in all the specimens of experimental groups in as few as 7 days. Reproducibility of the model was confirmed in a second investigation. Therefore, it was concluded that this model can be used for studying the microbial etiology and prevention of secondary caries.

KW - In vitro microbial model

KW - Lactobacillus casei

KW - Secondary caries

KW - Streptococcus mutans

UR - http://www.scopus.com/inward/record.url?scp=0030344526&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030344526&partnerID=8YFLogxK

M3 - Article

C2 - 8833134

AN - SCOPUS:0030344526

VL - 30

SP - 112

EP - 118

JO - Caries Research

JF - Caries Research

SN - 0008-6568

IS - 2

ER -