An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways

Naohiro Inohara, Takeyoshi Koseki, Jingmei Lin, Luis Del Peso, Peter C. Lucas, Felicia F. Chen, Yasunori Ogura, Gabriel Núñez

Research output: Contribution to journalArticle

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Abstract

Nod1 is an Apaf-1-like molecule composed of a caspase-recruitment domain (CARD), nucleotide-binding domain, and leucine-rich repeats that associates with the CARD-containing kinase RICK and activates nuclear factor κB (NF-κB). We show that self-association of Nod1 mediates proximity of RICK and the interaction of RICK with the γ subunit of the IκB kinase (IKKγ). Similarly, the RICK-related kinase RIP associated via its intermediate region with IKKγ. A mutant form of IKKγ deficient in binding to IKKα and IKKβ inhibited NF-κB activation induced by RICK or RIP. Enforced oligomerization of RICK or RIP as well as of IKKγ, IKKα, or IKKβ was sufficient for induction of NF-κB activation. Thus, the proximity of RICK, RIP, and IKK complexes may play an important role for NF-κB activation during Nod1 oligomerization or trimerization of the tumor necrosis factor α receptor.

Original languageEnglish (US)
Pages (from-to)27823-27831
Number of pages9
JournalJournal of Biological Chemistry
Volume275
Issue number36
DOIs
StatePublished - Sep 8 2000
Externally publishedYes

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Oligomerization
Phosphotransferases
Chemical activation
Caspases
Tumor Necrosis Factor Receptors
Leucine
Nucleotides
Association reactions
Molecules
Caspase Activation and Recruitment Domain

ASJC Scopus subject areas

  • Biochemistry

Cite this

Inohara, N., Koseki, T., Lin, J., Del Peso, L., Lucas, P. C., Chen, F. F., ... Núñez, G. (2000). An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways. Journal of Biological Chemistry, 275(36), 27823-27831. https://doi.org/10.1074/jbc.M003415200

An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways. / Inohara, Naohiro; Koseki, Takeyoshi; Lin, Jingmei; Del Peso, Luis; Lucas, Peter C.; Chen, Felicia F.; Ogura, Yasunori; Núñez, Gabriel.

In: Journal of Biological Chemistry, Vol. 275, No. 36, 08.09.2000, p. 27823-27831.

Research output: Contribution to journalArticle

Inohara, N, Koseki, T, Lin, J, Del Peso, L, Lucas, PC, Chen, FF, Ogura, Y & Núñez, G 2000, 'An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways', Journal of Biological Chemistry, vol. 275, no. 36, pp. 27823-27831. https://doi.org/10.1074/jbc.M003415200
Inohara, Naohiro ; Koseki, Takeyoshi ; Lin, Jingmei ; Del Peso, Luis ; Lucas, Peter C. ; Chen, Felicia F. ; Ogura, Yasunori ; Núñez, Gabriel. / An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 36. pp. 27823-27831.
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