An inhibitory role for the transcription factor Stat3 in controlling IL-4 and Bcl6 expression in follicular helper T cells

Hao Wu, Lin Lin Xu, Paulla Teuscher, Hong Liu, Mark H. Kaplan, Alexander L. Dent

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The transcription factor Bcl6 is required for development of follicular helper T (T<inf>FH</inf>) cells. Cytokines that activate Stat3 promote Bcl6 expression and T<inf>FH</inf> cell differentiation. Previous studies with an acute virus infection model showed that T<inf>FH</inf> cell differentiation was decreased but not blocked in the absence of Stat3. In this study, we further analyzed the role of Stat3 in T<inf>FH</inf> cells. In Peyer's patches, we found that compared with wild-type, Stat3-deficient T<inf>FH</inf> cells developed at a 25% lower rate and expressed increased IFN-γ and IL-4. Whereas Peyer's patch germinal center B cells developed at normal numbers with Stat3-deficient T<inf>FH</inf> cells, IgG1 class switching was greatly increased. Following immunization with sheep RBCs, splenic Stat3-deficient T<inf>FH</inf> cells developed at a slower rate than in control mice, and splenic germinal center B cells were markedly decreased. Stat3-deficient T<inf>FH</inf> cells developed poorly in a competitive bone marrow chimera environment. Under all conditions tested, Stat3-deficient T<inf>FH</inf> cells overexpressed both IL-4 and Bcl6, a pattern specific for the T<inf>FH</inf> cell population. Finally, we found in vitro that repression of IL-4 expression in CD4 T cells by Bcl6 required Stat3 function. Our data indicate that Stat3 can repress the expression of Bcl6 and IL-4 in T<inf>FH</inf> cells, and that Stat3 regulates the ability of Bcl6 to repress target genes. Overall, we conclude that Stat3 is required to fine-tune the expression of multiple key genes in T<inf>FH</inf> cells, and that the specific immune environment determines the function of Stat3 in T<inf>FH</inf> cells.

Original languageEnglish (US)
Pages (from-to)2080-2089
Number of pages10
JournalJournal of Immunology
Volume195
Issue number5
DOIs
StatePublished - Sep 1 2015

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Helper-Inducer T-Lymphocytes
Interleukin-4
Transcription Factors
Peyer's Patches
Germinal Center
Cell Differentiation
B-Lymphocytes
Immunoglobulin Class Switching
Virus Diseases
Genes
Immunization
Sheep
Immunoglobulin G
Bone Marrow
Cytokines
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

An inhibitory role for the transcription factor Stat3 in controlling IL-4 and Bcl6 expression in follicular helper T cells. / Wu, Hao; Xu, Lin Lin; Teuscher, Paulla; Liu, Hong; Kaplan, Mark H.; Dent, Alexander L.

In: Journal of Immunology, Vol. 195, No. 5, 01.09.2015, p. 2080-2089.

Research output: Contribution to journalArticle

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abstract = "The transcription factor Bcl6 is required for development of follicular helper T (TFH) cells. Cytokines that activate Stat3 promote Bcl6 expression and TFH cell differentiation. Previous studies with an acute virus infection model showed that TFH cell differentiation was decreased but not blocked in the absence of Stat3. In this study, we further analyzed the role of Stat3 in TFH cells. In Peyer's patches, we found that compared with wild-type, Stat3-deficient TFH cells developed at a 25{\%} lower rate and expressed increased IFN-γ and IL-4. Whereas Peyer's patch germinal center B cells developed at normal numbers with Stat3-deficient TFH cells, IgG1 class switching was greatly increased. Following immunization with sheep RBCs, splenic Stat3-deficient TFH cells developed at a slower rate than in control mice, and splenic germinal center B cells were markedly decreased. Stat3-deficient TFH cells developed poorly in a competitive bone marrow chimera environment. Under all conditions tested, Stat3-deficient TFH cells overexpressed both IL-4 and Bcl6, a pattern specific for the TFH cell population. Finally, we found in vitro that repression of IL-4 expression in CD4 T cells by Bcl6 required Stat3 function. Our data indicate that Stat3 can repress the expression of Bcl6 and IL-4 in TFH cells, and that Stat3 regulates the ability of Bcl6 to repress target genes. Overall, we conclude that Stat3 is required to fine-tune the expression of multiple key genes in TFH cells, and that the specific immune environment determines the function of Stat3 in TFH cells.",
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AU - Dent, Alexander L.

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AB - The transcription factor Bcl6 is required for development of follicular helper T (TFH) cells. Cytokines that activate Stat3 promote Bcl6 expression and TFH cell differentiation. Previous studies with an acute virus infection model showed that TFH cell differentiation was decreased but not blocked in the absence of Stat3. In this study, we further analyzed the role of Stat3 in TFH cells. In Peyer's patches, we found that compared with wild-type, Stat3-deficient TFH cells developed at a 25% lower rate and expressed increased IFN-γ and IL-4. Whereas Peyer's patch germinal center B cells developed at normal numbers with Stat3-deficient TFH cells, IgG1 class switching was greatly increased. Following immunization with sheep RBCs, splenic Stat3-deficient TFH cells developed at a slower rate than in control mice, and splenic germinal center B cells were markedly decreased. Stat3-deficient TFH cells developed poorly in a competitive bone marrow chimera environment. Under all conditions tested, Stat3-deficient TFH cells overexpressed both IL-4 and Bcl6, a pattern specific for the TFH cell population. Finally, we found in vitro that repression of IL-4 expression in CD4 T cells by Bcl6 required Stat3 function. Our data indicate that Stat3 can repress the expression of Bcl6 and IL-4 in TFH cells, and that Stat3 regulates the ability of Bcl6 to repress target genes. Overall, we conclude that Stat3 is required to fine-tune the expression of multiple key genes in TFH cells, and that the specific immune environment determines the function of Stat3 in TFH cells.

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