An interaction between α-actinin land the β1 integrin subunit in vitro

Carol A. Otey, Fredrick Pavalko, Keith Burridge

Research output: Contribution to journalArticle

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Abstract

A number of cytoskeletal-associated proteins that are concentrated in focal contacts, namely α-actinin, vinculin, talin, and integrin, have been shown to interact in vitro such that they suggest a potential link between actin filaments and the membrane. Because some of these interactions are of low affinity, we suspect that additional linkages also exist. Therefore, we have used a synthetic peptide corresponding to the cytoplasmic domain of β1 integrin and affinity chromatography to identify additional integrinbinding proteins. Here we report our finding of an interaction between the cytoplasmic domain of β1 integrin and the actin-binding protein α-actinin. β1-integrin cytoplasmic domain peptide columns bound several proteins from Triton extracts of chicken embryo fibroblasts. One protein at ∼100 kD was identified by immunoblot analysis as α-actinin. Solid phase binding assays indicated that α-actinin bound specifically and directly to the β1 peptide with relatively high affinity. Using purified heterodimeric chicken smooth muscle integrin (a β1 integrin) or the platelet integrin glycoprotein IIb/IIIa complex (a β3 integrin), binding of α-actinin was also observed in similar solid phase assays, albeit with a lower affinity than was seen using the β1 peptide. β-Actinin also bound specifically to phospholipid vesicles into which glycoprotein IIb/IIIa had been incorporated. These results lead us to suggest that this integrin-α-actinin linkage may contribute to the attachment of actin filaments to the membrane in certain locations.

Original languageEnglish (US)
Pages (from-to)721-729
Number of pages9
JournalJournal of Cell Biology
Volume111
Issue number2
StatePublished - Aug 1990
Externally publishedYes

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Actinin
Integrins
Platelet Glycoprotein GPIIb-IIIa Complex
Actin Cytoskeleton
Peptides
Chickens
Talin
Vinculin
Platelet Membrane Glycoproteins
In Vitro Techniques
Microfilament Proteins
Proteins
Focal Adhesions
Cytoskeletal Proteins
Membranes
Affinity Chromatography
Smooth Muscle
Phospholipids
Embryonic Structures
Fibroblasts

ASJC Scopus subject areas

  • Cell Biology

Cite this

An interaction between α-actinin land the β1 integrin subunit in vitro. / Otey, Carol A.; Pavalko, Fredrick; Burridge, Keith.

In: Journal of Cell Biology, Vol. 111, No. 2, 08.1990, p. 721-729.

Research output: Contribution to journalArticle

Otey, Carol A. ; Pavalko, Fredrick ; Burridge, Keith. / An interaction between α-actinin land the β1 integrin subunit in vitro. In: Journal of Cell Biology. 1990 ; Vol. 111, No. 2. pp. 721-729.
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