An IRP-like protein from Plasmodium falciparum binds to a mammalian iron-responsive element

Mark Loyevsky, Timothy LaVaute, Charles R. Allerson, Robert Stearman, Olakunle O. Kassim, Sharon Cooperman, Victor R. Gordeuk, Tracey A. Rouault

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

This study cloned and sequenced the complementary DNA (cDNA) encoding of a putative malarial iron responsive element-binding protein (PflRPa) and confirmed its identity to the previously identified iron-regulatory protein (IRP)-like cDNA from Plasmodium falciparum. Sequence alignment showed that the plasmodial sequence has 47% identity with human IRP1. Hemoglobin-free lysates obtained from erythrocyte-stage P falciparum contain a protein that binds a consensus mammalian iron-responsive element (IRE), indicating that a protein(s) with iron-regulatory activity was present in the lysates. IRE-binding activity was found to be iron regulated in the electrophoretic mobility shift assays. Western blot analysis showed a 2-fold increase in the level of PfIRPa in the desferrioxamine-treated cultures versus control or iron-supplemented cells. Malarial IRP was detected by anti-PfIRPa antibody in the IRE-protein complex from P falciparum lysates. Immunofluorescence studies confirmed the presence of PfIRPa in the infected red blood cells. These findings demonstrate that erythrocyte P falciparum contains an iron-regulated IRP that binds a mammalian consensus IRE sequence, raising the possibility that the malaria parasite expresses transcripts that contain IREs and are iron-dependently regulated.

Original languageEnglish (US)
Pages (from-to)2555-2562
Number of pages8
JournalBlood
Volume98
Issue number8
DOIs
StatePublished - Oct 15 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Loyevsky, M., LaVaute, T., Allerson, C. R., Stearman, R., Kassim, O. O., Cooperman, S., Gordeuk, V. R., & Rouault, T. A. (2001). An IRP-like protein from Plasmodium falciparum binds to a mammalian iron-responsive element. Blood, 98(8), 2555-2562. https://doi.org/10.1182/blood.V98.8.2555