An IRP-like protein from Plasmodium falciparum binds to a mammalian iron-responsive element

Mark Loyevsky, Timothy LaVaute, Charles R. Allerson, Robert Stearman, Olakunle O. Kassim, Sharon Cooperman, Victor R. Gordeuk, Tracey A. Rouault

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This study cloned and sequenced the complementary DNA (cDNA) encoding of a putative malarial iron responsive element-binding protein (PflRPa) and confirmed its identity to the previously identified iron-regulatory protein (IRP)-like cDNA from Plasmodium falciparum. Sequence alignment showed that the plasmodial sequence has 47% identity with human IRP1. Hemoglobin-free lysates obtained from erythrocyte-stage P falciparum contain a protein that binds a consensus mammalian iron-responsive element (IRE), indicating that a protein(s) with iron-regulatory activity was present in the lysates. IRE-binding activity was found to be iron regulated in the electrophoretic mobility shift assays. Western blot analysis showed a 2-fold increase in the level of PfIRPa in the desferrioxamine-treated cultures versus control or iron-supplemented cells. Malarial IRP was detected by anti-PfIRPa antibody in the IRE-protein complex from P falciparum lysates. Immunofluorescence studies confirmed the presence of PfIRPa in the infected red blood cells. These findings demonstrate that erythrocyte P falciparum contains an iron-regulated IRP that binds a mammalian consensus IRE sequence, raising the possibility that the malaria parasite expresses transcripts that contain IREs and are iron-dependently regulated.

Original languageEnglish (US)
Pages (from-to)2555-2562
Number of pages8
JournalBlood
Volume98
Issue number8
DOIs
StatePublished - Oct 15 2001
Externally publishedYes

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Iron-Regulatory Proteins
Plasmodium falciparum
Iron
Proteins
Erythrocytes
Complementary DNA
Electrophoretic mobility
Deferoxamine
Sequence Alignment
Electrophoretic Mobility Shift Assay
Malaria
Fluorescent Antibody Technique
Anti-Idiotypic Antibodies
Assays
Parasites
Hemoglobins
Blood
Western Blotting
Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Loyevsky, M., LaVaute, T., Allerson, C. R., Stearman, R., Kassim, O. O., Cooperman, S., ... Rouault, T. A. (2001). An IRP-like protein from Plasmodium falciparum binds to a mammalian iron-responsive element. Blood, 98(8), 2555-2562. https://doi.org/10.1182/blood.V98.8.2555

An IRP-like protein from Plasmodium falciparum binds to a mammalian iron-responsive element. / Loyevsky, Mark; LaVaute, Timothy; Allerson, Charles R.; Stearman, Robert; Kassim, Olakunle O.; Cooperman, Sharon; Gordeuk, Victor R.; Rouault, Tracey A.

In: Blood, Vol. 98, No. 8, 15.10.2001, p. 2555-2562.

Research output: Contribution to journalArticle

Loyevsky, M, LaVaute, T, Allerson, CR, Stearman, R, Kassim, OO, Cooperman, S, Gordeuk, VR & Rouault, TA 2001, 'An IRP-like protein from Plasmodium falciparum binds to a mammalian iron-responsive element', Blood, vol. 98, no. 8, pp. 2555-2562. https://doi.org/10.1182/blood.V98.8.2555
Loyevsky M, LaVaute T, Allerson CR, Stearman R, Kassim OO, Cooperman S et al. An IRP-like protein from Plasmodium falciparum binds to a mammalian iron-responsive element. Blood. 2001 Oct 15;98(8):2555-2562. https://doi.org/10.1182/blood.V98.8.2555
Loyevsky, Mark ; LaVaute, Timothy ; Allerson, Charles R. ; Stearman, Robert ; Kassim, Olakunle O. ; Cooperman, Sharon ; Gordeuk, Victor R. ; Rouault, Tracey A. / An IRP-like protein from Plasmodium falciparum binds to a mammalian iron-responsive element. In: Blood. 2001 ; Vol. 98, No. 8. pp. 2555-2562.
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