An isogenic hemoglobin receptor-deficient mutant of Haemophilus ducreyi is attenuated in the human model of experimental infection

Jaffar A. Al-Tawfiq, Kate R. Fortney, Barry Katz, Antoinette F. Hood, Christopher Elkins, Stanley Spinola

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Abstract

Haemophilus ducreyi expresses a conserved hemoglobin-binding outer- membrane protein (HgbA). To test the role of HgbA in pathogenesis, we infected 9 adults with isolate 35000 and its isogenic hgbA-inactivated mutant (FX504) on their upper arms in a double-blinded, escalating dose-response study. Papules developed at similar rates at sites inoculated with the mutant or parent. The pustule-formation rate was 55% (95% confidence interval [CI], 30.8%-78.5%) at parent sites and 0 (95% CI, 0-10.5%) at mutant sites (P < .0001). The recovery rate of H. ducreyi from surface cultures was 16% (n = 142) from parent sites and 0 (n = 213) from mutant sites (P < .0001). H. ducreyi was recovered at biopsy from 6 of 7 parent sites and from 0 of 3 mutant sites. The results indicate that hemoglobin may be a critical source of heme or iron for the establishment of H. ducreyi infection in humans.

Original languageEnglish
Pages (from-to)1049-1054
Number of pages6
JournalJournal of Infectious Diseases
Volume181
Issue number3
DOIs
StatePublished - 2000

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Haemophilus ducreyi
Hemoglobins
Theoretical Models
Infection
Haemophilus Infections
Confidence Intervals
Heme
Membrane Proteins
Arm
Iron
Biopsy

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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An isogenic hemoglobin receptor-deficient mutant of Haemophilus ducreyi is attenuated in the human model of experimental infection. / Al-Tawfiq, Jaffar A.; Fortney, Kate R.; Katz, Barry; Hood, Antoinette F.; Elkins, Christopher; Spinola, Stanley.

In: Journal of Infectious Diseases, Vol. 181, No. 3, 2000, p. 1049-1054.

Research output: Contribution to journalArticle

Al-Tawfiq, Jaffar A. ; Fortney, Kate R. ; Katz, Barry ; Hood, Antoinette F. ; Elkins, Christopher ; Spinola, Stanley. / An isogenic hemoglobin receptor-deficient mutant of Haemophilus ducreyi is attenuated in the human model of experimental infection. In: Journal of Infectious Diseases. 2000 ; Vol. 181, No. 3. pp. 1049-1054.
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AU - Elkins, Christopher

AU - Spinola, Stanley

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