An unusual transposon encoding heat shock inducible and developmentally regulated transcripts in Dictyostelium

Elliot Rosen, Annegrethe Sivertsen, Richard A. Firtel

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Abstract

We have isolated and characterized a transposable element from Dictyostelium discoideum denoted Tdd-1. There are ∼50 complete copies of the element per haploid genome and ∼100-150 partial elements. Southern blots of DNA from different Dictyostelium discoideum strains show that Tdd-1 is a mobile element. Tdd-1 is 4.9 kb long, with 313 bp inverted repeats. These repeats lie near the termini, but unlike other transposable elements one end of Tdd-1 extends 36 bp past the repeat and the other extends 1 bp. Tdd-1 encodes a series of developmentally regulated transcripts, all with the same polarity, that increase dramatically in abundance after ∼10 hr of development. We have also identified a heat shock inducible transcript of the opposite polarity.

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalCell
Volume35
Issue number1
DOIs
StatePublished - Nov 1983

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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