An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies

N. Leigh Anderson, R. Esquer-Blasco, J. P. Hofmann, L. Meheus, J. Raymackers, S. Steiner, Frank Witzmann, N. G. Anderson

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We have improved upon the reference two-dimensional (2-D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907-930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2-D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high-performance liquid chromatography (HPLC), were sequenced using a Perkin-Elmer 477A sequenator. Additional spots were identified using specific antibodies.

Original languageEnglish (US)
Pages (from-to)1977-1981
Number of pages5
JournalElectrophoresis
Volume16
Issue number10
StatePublished - 1995

Fingerprint

Drug and Narcotic Control
Gene expression
Liver
Rats
Gels
Databases
High performance liquid chromatography
Electrophoresis
Pharmaceutical Preparations
Genes
Digestion
Proteins
High Pressure Liquid Chromatography
Peptides
Antibodies
Coomassie blue

Keywords

  • Calibration
  • Identification
  • Liver
  • Map
  • Two-dimensional polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Leigh Anderson, N., Esquer-Blasco, R., Hofmann, J. P., Meheus, L., Raymackers, J., Steiner, S., ... Anderson, N. G. (1995). An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies. Electrophoresis, 16(10), 1977-1981.

An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies. / Leigh Anderson, N.; Esquer-Blasco, R.; Hofmann, J. P.; Meheus, L.; Raymackers, J.; Steiner, S.; Witzmann, Frank; Anderson, N. G.

In: Electrophoresis, Vol. 16, No. 10, 1995, p. 1977-1981.

Research output: Contribution to journalArticle

Leigh Anderson, N, Esquer-Blasco, R, Hofmann, JP, Meheus, L, Raymackers, J, Steiner, S, Witzmann, F & Anderson, NG 1995, 'An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies', Electrophoresis, vol. 16, no. 10, pp. 1977-1981.
Leigh Anderson N, Esquer-Blasco R, Hofmann JP, Meheus L, Raymackers J, Steiner S et al. An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies. Electrophoresis. 1995;16(10):1977-1981.
Leigh Anderson, N. ; Esquer-Blasco, R. ; Hofmann, J. P. ; Meheus, L. ; Raymackers, J. ; Steiner, S. ; Witzmann, Frank ; Anderson, N. G. / An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies. In: Electrophoresis. 1995 ; Vol. 16, No. 10. pp. 1977-1981.
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