We have improved upon the reference two‐dimensional (2‐D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907–930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2‐D gels were submitted to internal tryptic digestion , and individual peptides, separated by high‐performance liquid chromatography (HPLC), were sequenced using a Perkin‐Elmer 477A sequenator. Additional spots were identified using specific antibodies.
- Two‐dimensional polyacrylamide gel electrophoresis
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry