An updated two‐dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies

N. Leigh Anderson, Ricardo Esquer‐Blasco, Jean‐Paul ‐P Hofmann, Lydie Meheus, Jos Raymackers, Sandra Steiner, Frank Witzmann, Norman G. Anderson

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Abstract

We have improved upon the reference two‐dimensional (2‐D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907–930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2‐D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high‐performance liquid chromatography (HPLC), were sequenced using a Perkin‐Elmer 477A sequenator. Additional spots were identified using specific antibodies.

Original languageEnglish (US)
Pages (from-to)1977-1981
Number of pages5
JournalELECTROPHORESIS
Volume16
Issue number1
DOIs
StatePublished - 1995

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Keywords

  • Calibration
  • Identification
  • Liver
  • Map
  • Two‐dimensional polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Anderson, N. L., Esquer‐Blasco, R., Hofmann, JP. P., Meheus, L., Raymackers, J., Steiner, S., Witzmann, F., & Anderson, N. G. (1995). An updated two‐dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies. ELECTROPHORESIS, 16(1), 1977-1981. https://doi.org/10.1002/elps.11501601324