Anabolic effects of human biosynthetic parathyroid hormone fragment (1- 34), LY333334, on remodeling and mechanical properties of cortical bone in rabbits

Toru Hirano, David Burr, Charles H. Turner, Masahiko Sato, Rick L. Cain, Janet M. Hock

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Intermittent administration of parathyroid hormone (PTH) has an anabolic effect in cancellous bone of osteoporotic humans. However, the effect of PTH on cortical bone with Haversian remodeling remains controversial. The aim of this study was to determine the effects of biosynthetic human PTH(1-34) on the histology and mechanical properties of cortical bone in rabbits, which exhibit Haversian remodeling. Mature New Zealand white rabbits were treated with once daily injections of vehicle, or PTH(1-34), LY333334, at 10 μg/kg/day or 40 μg/kg/day for 140 days. Body weight in rabbits treated with PTH did not change significantly over the experimental period. Serum calcium and phosphate were within the normal range, but a 1 mg/ml increase in serum calcium was observed in rabbits given the higher dose of PTH. Histomorphometry of cortical bone in the midshaft of the tibia showed significant increases in periosteal and endocortical bone formation in these rabbits. Intracortical bone remodeling in the tibia was activated and cortical porosity increased by PTH. Cross-sectional bone area and bone mass of the midshaft of the femur increased significantly after PTH treatment. Ultimate force, stiffness, and work to failure of the midshaft of the femur of rabbits given the 40 μg dose of PTH were significantly greater than those in the control group, whereas elastic modulus was significantly lower than that in the rabbits given the 10 μg dose of PTH, but not different from controls. In the third lumbar vertebra, PTH increased both formation and resorption without increasing cancellous bone volume. The increases in bone turnover and cortical porosity were accompanied by concurrent increases in bone at the periosteal and endocortical surfaces. The combination of these phenomena resulted in an enhancement of the ultimate stress, stiffness, and work to failure of the femur.

Original languageEnglish
Pages (from-to)536-545
Number of pages10
JournalJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Volume14
Issue number4
StatePublished - 1999

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Anabolic Agents
Parathyroid Hormone
Rabbits
Femur
Bone Remodeling
Porosity
Tibia
Cortical Bone
human PTH protein
Teriparatide
Bone and Bones
Lumbar Vertebrae
Elastic Modulus
Serum
Osteogenesis
Histology
Reference Values
Body Weight
Calcium

ASJC Scopus subject areas

  • Surgery

Cite this

Anabolic effects of human biosynthetic parathyroid hormone fragment (1- 34), LY333334, on remodeling and mechanical properties of cortical bone in rabbits. / Hirano, Toru; Burr, David; Turner, Charles H.; Sato, Masahiko; Cain, Rick L.; Hock, Janet M.

In: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 14, No. 4, 1999, p. 536-545.

Research output: Contribution to journalArticle

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