Analyses of Endothelial Cells and Endothelial Progenitor Cells Released Microvesicles by Using Microbead and Q-dot Based Nanoparticle Tracking Analysis

Jinju Wang, Yun Zhong, Xiaotang Ma, Xiang Xiao, Chuanfang Cheng, Yusen Chen, Ifeanyi Iwuchukwu, Kenneth J. Gaines, Zhao Bin Zhao, Shiming Liu, Jeffrey Travers, Ji C. Bihl, Yanfang Chen

Research output: Contribution to journalArticle

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Abstract

Accurate analysis of specific microvesicles (MVs) from biofluids is critical and challenging. Here we described novel methods to purify and detect MVs shed from endothelial cells (ECs) and endothelial progenitor cells (EPCs) by combining microbeads with fluorescence quantum dots (Q-dots) coupled nanoparticle tracking analysis (NTA). In the in vitro screening systems, we demonstrated that 1) anti-CD105 (EC marker) and anti-CD34 (EPC marker) conjugated-microbeads had the highest sensitivity and specificity for isolating respective MVs, which were confirmed with negative controls, CD41 and CD235a; 2) anti-CD144 (EC marker) and anti-KDR (EPC marker) conjugated-Q-dots exhibited the best sensitivity and specificity for their respective MV NTA detection, which were confirmed with positive control, anti-Annexin V (MV universal marker). The methods were further validated by their ability to efficiently recover the known amount of EC-MVs and EPC-MVs from particle-depleted plasma, and to detect the dynamical changes of plasma MVs in ischemic stroke patients, as compared with traditional flow cytometry. These novel methods provide ideal approaches for functional analysis and biomarker discovery of ECs-and EPCs-derived MVs.

Original languageEnglish (US)
Article number24679
JournalScientific Reports
Volume6
DOIs
StatePublished - Apr 20 2016
Externally publishedYes

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Quantum Dots
Microspheres
Nanoparticles
Endothelial Cells
Sensitivity and Specificity
Annexin A5
Flow Cytometry
Biomarkers
Fluorescence
Stroke
Endothelial Progenitor Cells

ASJC Scopus subject areas

  • General

Cite this

Analyses of Endothelial Cells and Endothelial Progenitor Cells Released Microvesicles by Using Microbead and Q-dot Based Nanoparticle Tracking Analysis. / Wang, Jinju; Zhong, Yun; Ma, Xiaotang; Xiao, Xiang; Cheng, Chuanfang; Chen, Yusen; Iwuchukwu, Ifeanyi; Gaines, Kenneth J.; Bin Zhao, Zhao; Liu, Shiming; Travers, Jeffrey; Bihl, Ji C.; Chen, Yanfang.

In: Scientific Reports, Vol. 6, 24679, 20.04.2016.

Research output: Contribution to journalArticle

Wang, J, Zhong, Y, Ma, X, Xiao, X, Cheng, C, Chen, Y, Iwuchukwu, I, Gaines, KJ, Bin Zhao, Z, Liu, S, Travers, J, Bihl, JC & Chen, Y 2016, 'Analyses of Endothelial Cells and Endothelial Progenitor Cells Released Microvesicles by Using Microbead and Q-dot Based Nanoparticle Tracking Analysis', Scientific Reports, vol. 6, 24679. https://doi.org/10.1038/srep24679
Wang, Jinju ; Zhong, Yun ; Ma, Xiaotang ; Xiao, Xiang ; Cheng, Chuanfang ; Chen, Yusen ; Iwuchukwu, Ifeanyi ; Gaines, Kenneth J. ; Bin Zhao, Zhao ; Liu, Shiming ; Travers, Jeffrey ; Bihl, Ji C. ; Chen, Yanfang. / Analyses of Endothelial Cells and Endothelial Progenitor Cells Released Microvesicles by Using Microbead and Q-dot Based Nanoparticle Tracking Analysis. In: Scientific Reports. 2016 ; Vol. 6.
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