Analysis of a sequenced cDNA library from multiple sclerosis lesions

Kevin G. Becker, David Mattson, James M. Powers, Ameer M. Gado, William E. Biddison

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

To identify genes that are expressed in MS pathogenesis, we have analyzed a normalized cDNA library made from mRNA obtained from CNS lesions of a patient with primary progressive MS. Complementary DNA clones obtained from this library were subjected to automated DNA sequencing to generate expressed sequence tags. Analysis of this MS cDNA library revealed the presence of 54 cDNAs that were associated with immune activation and indicated the presence of an ongoing inflammatory response with evidence of both cell-mediated and humoral immune responses. The surprising finding was that 16 of the cDNAs encoded autoantigens associated with seven other autoimmune disorders, while only three of these 16 autoantigen cDNAs were present in a similarly constructed adult brain library. Such aberrant autoantigen expression could provide a source of secondary autoimmune stimulation that could contribute to the ongoing inflammatory response in MS. In addition, two cDNAs were found that mapped to a known MS susceptibility locus (5p14-p12): one encoded an excitatory amino acid transporter and the other a human homologue of the Drosophila disabled gene. This approach to the molecular biology of MS pathogenesis may help to illuminate previously unappreciated aspects of this disease.

Original languageEnglish (US)
Pages (from-to)27-38
Number of pages12
JournalJournal of Neuroimmunology
Volume77
Issue number1
DOIs
StatePublished - Jul 1997
Externally publishedYes

Fingerprint

Gene Library
Multiple Sclerosis
Complementary DNA
Autoantigens
Amino Acid Transport Systems
Excitatory Amino Acids
Expressed Sequence Tags
Humoral Immunity
DNA Sequence Analysis
Genes
Libraries
Drosophila
Molecular Biology
Clone Cells
Messenger RNA
Brain

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Analysis of a sequenced cDNA library from multiple sclerosis lesions. / Becker, Kevin G.; Mattson, David; Powers, James M.; Gado, Ameer M.; Biddison, William E.

In: Journal of Neuroimmunology, Vol. 77, No. 1, 07.1997, p. 27-38.

Research output: Contribution to journalArticle

Becker, Kevin G. ; Mattson, David ; Powers, James M. ; Gado, Ameer M. ; Biddison, William E. / Analysis of a sequenced cDNA library from multiple sclerosis lesions. In: Journal of Neuroimmunology. 1997 ; Vol. 77, No. 1. pp. 27-38.
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