Analysis of apoptosis: Basic principles and protocols

Man Jiang, Craig Brooks, Guie Dong, Xiaoning Li, Hong Min Ni, Xiao Ming Yin, Zheng Dong

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Apoptosis is a distinct form of cell demise. Originally defined by cellular morphology, apoptosis can now be characterized at the molecular, biochemical, and cellular levels. The detection of apoptosis has become more important, not only in the interests of scientific investigation, but also because of its significance in clinical practice. For example, since apoptosis has been implicated in a variety of devastating diseases such as cancer, therapies targeting apoptosis are being developed. To evaluate the effectiveness of the treatment, one would have to assess the apoptotic response before, during, and after the therapy. For the typical apoptosis, a set of characteristics in cell structure and biochemistry has been well defined. In combination, these provide the basis for apoptosis detection in a given setting. The methodology for analyzing these characteristics is as diverse as the research subjects. Several books devoted to the methodology of apoptosis analysis have recently been published (1-3). Readers may find detailed experimental protocols in these books. This chapter provides an overview of the basic approaches used in analyzing apoptosis, the principle and the basic methodology, in order to provide a quick guide for readers to use when deciding which methods are available and appropriate for their own study. We start with the determination of cell viability and the morphology of dying cells. We then discuss approaches for examining apoptotic changes on the cell membrane, in the cytosol, and in the nucleus. We also summarize some of the common sources of reagents for apoptosis research.

Original languageEnglish (US)
Title of host publicationEssentials of Apoptosis
Subtitle of host publicationA Guide for Basic and Clinical Research
PublisherHumana Press Inc.
Pages691-711
Number of pages21
ISBN (Electronic)9781603273817
ISBN (Print)9781603273800
DOIs
StatePublished - Jan 1 2009

Keywords

  • Caspase
  • Cell viability
  • Microscopy
  • Mitochondria
  • TUNEL assay

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Jiang, M., Brooks, C., Dong, G., Li, X., Ni, H. M., Yin, X. M., & Dong, Z. (2009). Analysis of apoptosis: Basic principles and protocols. In Essentials of Apoptosis: A Guide for Basic and Clinical Research (pp. 691-711). Humana Press Inc.. https://doi.org/10.1007/978-1-60327-381-7_31