Analysis of cDNA sequences of feline SAAs

Maartje Van Rossum, Fons J A M Van Asten, Jaime Rofina, Johannes A. Lenstra, Merrill Benson, Erik Gruys

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Feline amyloidosis is an uncommon disorder caused by deposits of amyloid in a variety of organs. Most frequently encountered types are amyloid derived of pancreatic islet amyloid polypeptide (AIAPP) in older cats and of the apolipoprotein, apo-serum amyloid A (AA) in Abyssinian/Somali (Aby) and Siamese/Oriental (Siam) cats occurring at a relatively young age. For the AA protein of the Aby, Siam and domestic shorthair cat (DSH) breed different amino acid sequences have been described. It is not yet clear, however, whether the tendency to develop AA amyloidosis in Aby and Siam is associated with specific apoSAA protein sequences and whether this is breed specific. In this study, DNA from one Siam and two DSH cats revealed on Southern blot three bands suggesting at least three genes or gene clusters. The SAA cDNAs of hepatic mRNA from three Abys, five Siams and five DSHs were amplified by RT-PCR, cloned and sequenced. Siams and Abys had limited SAA sequence variability. All five Siams, three of which were positive for amyloid, had the amyloidogenic Siam SAA and the amyloidogenic Aby SAA sequence. Two of the Abys, both with amyloid, had the amyloidogenic Aby SAA sequence. The third Aby, without amyloid, missed its amyloidogenic sequence. The SAA sequences of the DSHs found in the present preliminary survey, suggested a possible tendency for more variability, whereas the amyloidogenic Siam as well as the amyloidogenic Aby sequence were found once. Up to five different sequences were found in a single animal. All five DSHs, moreover, had a specific sequence lacking in the Siams and Abys. The present results, especially those of the Siams, favor that in addition to the occurrence of amyloid associated SAA genes other factors such as infections and inflammatory processes are involved in the development of phenotypical amyloidosis.

Original languageEnglish
Pages (from-to)38-43
Number of pages6
JournalAmyloid
Volume11
Issue number1
DOIs
StatePublished - Mar 2004

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Felidae
Amyloid
Sequence Analysis
Complementary DNA
Cats
Amyloidosis
Serum Amyloid A Protein
Islet Amyloid Polypeptide
Pancreatic Polypeptide
Apolipoproteins
Amyloid Plaques
Multigene Family
Southern Blotting
Islets of Langerhans
Genes
Amino Acid Sequence
Polymerase Chain Reaction
Messenger RNA
Liver
DNA

Keywords

  • Abyssinian cat
  • Amino acid sequence
  • Domestic shorthair cat
  • Serum amyloid A
  • Siamese cat

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Van Rossum, M., Van Asten, F. J. A. M., Rofina, J., Lenstra, J. A., Benson, M., & Gruys, E. (2004). Analysis of cDNA sequences of feline SAAs. Amyloid, 11(1), 38-43. https://doi.org/10.1080/13506120410001676836

Analysis of cDNA sequences of feline SAAs. / Van Rossum, Maartje; Van Asten, Fons J A M; Rofina, Jaime; Lenstra, Johannes A.; Benson, Merrill; Gruys, Erik.

In: Amyloid, Vol. 11, No. 1, 03.2004, p. 38-43.

Research output: Contribution to journalArticle

Van Rossum, M, Van Asten, FJAM, Rofina, J, Lenstra, JA, Benson, M & Gruys, E 2004, 'Analysis of cDNA sequences of feline SAAs', Amyloid, vol. 11, no. 1, pp. 38-43. https://doi.org/10.1080/13506120410001676836
Van Rossum M, Van Asten FJAM, Rofina J, Lenstra JA, Benson M, Gruys E. Analysis of cDNA sequences of feline SAAs. Amyloid. 2004 Mar;11(1):38-43. https://doi.org/10.1080/13506120410001676836
Van Rossum, Maartje ; Van Asten, Fons J A M ; Rofina, Jaime ; Lenstra, Johannes A. ; Benson, Merrill ; Gruys, Erik. / Analysis of cDNA sequences of feline SAAs. In: Amyloid. 2004 ; Vol. 11, No. 1. pp. 38-43.
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