Analysis of Electrostatic Interactions and Their Relationship to Conformation and Stability of Bovine Pancreatic Trypsin Inhibitor

Keith L. March, David G. Maskalick, Richard D. England, Stephen H. Friend, Frank R.N. Gurd

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The modified Tanford-Kirkwood electrostatic theory has been employed to evaluate pK values for all charge sites in the bovine pancreatic trypsin inhibitor (BPTI). 13C NMR titration data were obtained for all titrating groups except arginine residues in BPTI at nearly constant ionic strength in 0.1 M NaCl, at 41°C. The chemical shifts of 46 resonances were found to be sensitive to pH. The pK values of these titrating resonances compared well with those computed by the modified Tanford-Kirkwood electrostatic theory. A conformational change involving the NH2- and COOH-terminal and nearby residues is shown to be partly electrostatically driven by the formation of a salt bridge between the α-amino and α-carboxyl groups at mid-pH values. The computed total electrostatic free energy of the molecule is found to be stabilizing at neutral pH despite the substantial net positive charge borne by the protein under such conditions.

Original languageEnglish (US)
Pages (from-to)5241-5251
Number of pages11
JournalBiochemistry
Volume21
Issue number21
DOIs
StatePublished - Jan 1 1982

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Aprotinin
Coulomb interactions
Static Electricity
Conformations
Electrostatics
Chemical shift
Ionic strength
Titration
Osmolar Concentration
Free energy
Arginine
Salts
Nuclear magnetic resonance
Molecules
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Analysis of Electrostatic Interactions and Their Relationship to Conformation and Stability of Bovine Pancreatic Trypsin Inhibitor. / March, Keith L.; Maskalick, David G.; England, Richard D.; Friend, Stephen H.; Gurd, Frank R.N.

In: Biochemistry, Vol. 21, No. 21, 01.01.1982, p. 5241-5251.

Research output: Contribution to journalArticle

March, Keith L. ; Maskalick, David G. ; England, Richard D. ; Friend, Stephen H. ; Gurd, Frank R.N. / Analysis of Electrostatic Interactions and Their Relationship to Conformation and Stability of Bovine Pancreatic Trypsin Inhibitor. In: Biochemistry. 1982 ; Vol. 21, No. 21. pp. 5241-5251.
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