Analysis of fatty acids in mouse tissue via in situ transmethylation with biochar

Jechan Lee, Yiu Fai Tsang, Jeong Ik Oh, Seokmann Hong, Changsung Kim, Eilhann E. Kwon

Research output: Contribution to journalArticle

Abstract

Lipid derivatization technology-mediated fatty acid profiling studies have been suggested to dissect the contents of lipids in white fat and brown fat tissue. The focus of this study is to profile fatty acid lipidomics in brown adipose tissue and white adipose tissue of mice by derivatizing their lipids into fatty acid methyl esters via in situ transmethylation using a rice husk-derived biochar as porous media. The in situ transmethylation using biochar is advantageous in biological analysis because there was no loss of samples inevitably occurring in the loss of lipid in solvent extraction and purification steps.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalEnvironmental Geochemistry and Health
DOIs
StateAccepted/In press - Apr 28 2017
Externally publishedYes

Fingerprint

Fatty acids
Lipids
Fatty Acids
fatty acid
lipid
Tissue
Oils and fats
fat
Fats
biological analysis
Solvent extraction
ester
Purification
purification
Porous materials
porous medium
Esters
rice
in situ
analysis

Keywords

  • Biochar application
  • Derivatization
  • Lipid profiling
  • Tissue
  • Transmethylation

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Water Science and Technology
  • Environmental Science(all)
  • Geochemistry and Petrology

Cite this

Analysis of fatty acids in mouse tissue via in situ transmethylation with biochar. / Lee, Jechan; Tsang, Yiu Fai; Oh, Jeong Ik; Hong, Seokmann; Kim, Changsung; Kwon, Eilhann E.

In: Environmental Geochemistry and Health, 28.04.2017, p. 1-6.

Research output: Contribution to journalArticle

Lee, Jechan ; Tsang, Yiu Fai ; Oh, Jeong Ik ; Hong, Seokmann ; Kim, Changsung ; Kwon, Eilhann E. / Analysis of fatty acids in mouse tissue via in situ transmethylation with biochar. In: Environmental Geochemistry and Health. 2017 ; pp. 1-6.
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