Analysis of human gliomas by swab touch spray-mass spectrometry

Applications to intraoperative assessment of surgical margins and presence of oncometabolites

Valentina Pirro, Raquel Sero Llor, Alan K. Jarmusch, Clint M. Alfaro, Aaron Cohen-Gadol, Eyas M. Hattab, R. Graham Cooks

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Touch spray mass spectrometry using medical swabs is an ambient ionization technique (ionization of unprocessed sample in the open air) that has potential intraoperative application in quickly identifying the disease state of tissue and in better characterizing the resection margin. To explore this potential, we studied 29 human brain tumor specimens and obtained evidence that this technique can provide diagnostic molecular information that is relevant to brain cancer. Touch spray using medical swabs involves the physical sampling of tissue using a medical swab on a spatial scale of a few mm2 with subsequent ionization occurring directly from the swab tip upon addition of solvent and application of a high voltage. Using a tertiary mixture of acetonitrile, N,N-dimethylformamide, and ethanol, membrane-derived phospholipids and oncometabolites are extracted from the tissue, incorporated into the sprayed microdroplets, vacuumed into the mass spectrometer, and characterized in the resulting mass spectra. The tumor cell load was assessed from the complex phospholipid pattern in the mass spectra and also separately by measurement of N-acetylaspartate. Mutation status of the isocitrate dehydrogenase gene was determined via detection of the oncometabolite 2-hydroxyglutarate. The lack of sample pretreatment makes touch spray mass spectrometry using medical swabs a feasible intraoperative strategy for rapid surgical assessment.

Original languageEnglish (US)
Pages (from-to)4058-4066
Number of pages9
JournalAnalyst
Volume142
Issue number21
DOIs
StatePublished - Nov 7 2017

Fingerprint

Touch
Glioma
spray
Ionization
Mass spectrometry
Mass Spectrometry
ionization
mass spectrometry
Phospholipids
Tissue
phospholipid
Brain Neoplasms
tumor
brain
Tumors
Brain
Isocitrate Dehydrogenase
Dimethylformamide
Molecular Pathology
Mass spectrometers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Analysis of human gliomas by swab touch spray-mass spectrometry : Applications to intraoperative assessment of surgical margins and presence of oncometabolites. / Pirro, Valentina; Llor, Raquel Sero; Jarmusch, Alan K.; Alfaro, Clint M.; Cohen-Gadol, Aaron; Hattab, Eyas M.; Cooks, R. Graham.

In: Analyst, Vol. 142, No. 21, 07.11.2017, p. 4058-4066.

Research output: Contribution to journalArticle

Pirro, Valentina ; Llor, Raquel Sero ; Jarmusch, Alan K. ; Alfaro, Clint M. ; Cohen-Gadol, Aaron ; Hattab, Eyas M. ; Cooks, R. Graham. / Analysis of human gliomas by swab touch spray-mass spectrometry : Applications to intraoperative assessment of surgical margins and presence of oncometabolites. In: Analyst. 2017 ; Vol. 142, No. 21. pp. 4058-4066.
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