Analysis of lysophospholipids in human body fluids

Comparison of the lysophospholipid content in malignant vs. nonmalignant diseases

Yi Jin Xiao, Yan Xu

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Qualitative and quantitative analyses of lysophospholipids (lyso-PLs) in human body fluids and tissues have become critically important for our understanding of human physiology and pathology. As a new class of signaling molecules, these lipids play important regulatory roles in cellular functions. Thus, it is not surprising to find that a number of lyso-PLs are potential biomarkers for a variety of diseases, including cancers. Traditional lipid analytical methods, such as thin-layer chromatography (TLC), phosphorous determination, gas chromatography (GC), and high-performance liquid chromatography (HPLC) have been successfully used in phospholipid analysis. However, due to either the lack of sensitivity or the cumbersome analytical procedures, these methods are not highly effective in analyzing most lyso-PLs, some of which compose <1% of the total phospholipids. In addition, a complex array of enzymes and their regulators are involved in the metabolism of lyso-PLs. Therefore, analysis of lyso-PLs in human body fluids and tissues is not only an analytical, but also a biological issue. A good understanding of the biology of these lipids is essential for pathophysiologically relevant analyses of lyso-PLs. We will focus our discussion on: (1) a comparison of the electrospray ionization mass spectrometry (ESI-MS)-based method with other methods of lyso-PL analysis; (2) the influences of sample preparation on the analyses results; and (3) the issues related to sensitivity and reproducibility of the analyses, which are extremely important for biomarker identification and potential clinical use. The chapter includes discussions on the methods for qualitative and quantitative analysis of lyso-PLs in human body fluids (plasma, ascitic fluid, and urine) and in cells or cell culture media, as well as in animal tissues. The important factors that significantly affect the assays are emphasized. As future perspectives, we discuss the importance of further increasing the sensitivity and accuracy of the lyso-PL assays, as well as potential methods to establish high-throughput assays for lyso-PLs.

Original languageEnglish (US)
Title of host publicationFunctional Lipidomics
PublisherCRC Press
Pages125-145
Number of pages21
ISBN (Electronic)9781420027655
ISBN (Print)9781574444674
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Lysophospholipids
body fluids
Body fluids
Body Fluids
Human Body
Lipids
Assays
Phospholipids
Biomarkers
Tissue
Thin layer chromatography
Electrospray ionization
Physiology
High performance liquid chromatography
Pathology
biomarkers
phospholipids
Cell culture
Metabolism
assays

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Agricultural and Biological Sciences(all)
  • Medicine(all)

Cite this

Analysis of lysophospholipids in human body fluids : Comparison of the lysophospholipid content in malignant vs. nonmalignant diseases. / Xiao, Yi Jin; Xu, Yan.

Functional Lipidomics. CRC Press, 2005. p. 125-145.

Research output: Chapter in Book/Report/Conference proceedingChapter

@inbook{d590277d996247daab2bc327e798ed46,
title = "Analysis of lysophospholipids in human body fluids: Comparison of the lysophospholipid content in malignant vs. nonmalignant diseases",
abstract = "Qualitative and quantitative analyses of lysophospholipids (lyso-PLs) in human body fluids and tissues have become critically important for our understanding of human physiology and pathology. As a new class of signaling molecules, these lipids play important regulatory roles in cellular functions. Thus, it is not surprising to find that a number of lyso-PLs are potential biomarkers for a variety of diseases, including cancers. Traditional lipid analytical methods, such as thin-layer chromatography (TLC), phosphorous determination, gas chromatography (GC), and high-performance liquid chromatography (HPLC) have been successfully used in phospholipid analysis. However, due to either the lack of sensitivity or the cumbersome analytical procedures, these methods are not highly effective in analyzing most lyso-PLs, some of which compose <1{\%} of the total phospholipids. In addition, a complex array of enzymes and their regulators are involved in the metabolism of lyso-PLs. Therefore, analysis of lyso-PLs in human body fluids and tissues is not only an analytical, but also a biological issue. A good understanding of the biology of these lipids is essential for pathophysiologically relevant analyses of lyso-PLs. We will focus our discussion on: (1) a comparison of the electrospray ionization mass spectrometry (ESI-MS)-based method with other methods of lyso-PL analysis; (2) the influences of sample preparation on the analyses results; and (3) the issues related to sensitivity and reproducibility of the analyses, which are extremely important for biomarker identification and potential clinical use. The chapter includes discussions on the methods for qualitative and quantitative analysis of lyso-PLs in human body fluids (plasma, ascitic fluid, and urine) and in cells or cell culture media, as well as in animal tissues. The important factors that significantly affect the assays are emphasized. As future perspectives, we discuss the importance of further increasing the sensitivity and accuracy of the lyso-PL assays, as well as potential methods to establish high-throughput assays for lyso-PLs.",
author = "Xiao, {Yi Jin} and Yan Xu",
year = "2005",
month = "1",
day = "1",
doi = "10.1201/9781420027655",
language = "English (US)",
isbn = "9781574444674",
pages = "125--145",
booktitle = "Functional Lipidomics",
publisher = "CRC Press",

}

TY - CHAP

T1 - Analysis of lysophospholipids in human body fluids

T2 - Comparison of the lysophospholipid content in malignant vs. nonmalignant diseases

AU - Xiao, Yi Jin

AU - Xu, Yan

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Qualitative and quantitative analyses of lysophospholipids (lyso-PLs) in human body fluids and tissues have become critically important for our understanding of human physiology and pathology. As a new class of signaling molecules, these lipids play important regulatory roles in cellular functions. Thus, it is not surprising to find that a number of lyso-PLs are potential biomarkers for a variety of diseases, including cancers. Traditional lipid analytical methods, such as thin-layer chromatography (TLC), phosphorous determination, gas chromatography (GC), and high-performance liquid chromatography (HPLC) have been successfully used in phospholipid analysis. However, due to either the lack of sensitivity or the cumbersome analytical procedures, these methods are not highly effective in analyzing most lyso-PLs, some of which compose <1% of the total phospholipids. In addition, a complex array of enzymes and their regulators are involved in the metabolism of lyso-PLs. Therefore, analysis of lyso-PLs in human body fluids and tissues is not only an analytical, but also a biological issue. A good understanding of the biology of these lipids is essential for pathophysiologically relevant analyses of lyso-PLs. We will focus our discussion on: (1) a comparison of the electrospray ionization mass spectrometry (ESI-MS)-based method with other methods of lyso-PL analysis; (2) the influences of sample preparation on the analyses results; and (3) the issues related to sensitivity and reproducibility of the analyses, which are extremely important for biomarker identification and potential clinical use. The chapter includes discussions on the methods for qualitative and quantitative analysis of lyso-PLs in human body fluids (plasma, ascitic fluid, and urine) and in cells or cell culture media, as well as in animal tissues. The important factors that significantly affect the assays are emphasized. As future perspectives, we discuss the importance of further increasing the sensitivity and accuracy of the lyso-PL assays, as well as potential methods to establish high-throughput assays for lyso-PLs.

AB - Qualitative and quantitative analyses of lysophospholipids (lyso-PLs) in human body fluids and tissues have become critically important for our understanding of human physiology and pathology. As a new class of signaling molecules, these lipids play important regulatory roles in cellular functions. Thus, it is not surprising to find that a number of lyso-PLs are potential biomarkers for a variety of diseases, including cancers. Traditional lipid analytical methods, such as thin-layer chromatography (TLC), phosphorous determination, gas chromatography (GC), and high-performance liquid chromatography (HPLC) have been successfully used in phospholipid analysis. However, due to either the lack of sensitivity or the cumbersome analytical procedures, these methods are not highly effective in analyzing most lyso-PLs, some of which compose <1% of the total phospholipids. In addition, a complex array of enzymes and their regulators are involved in the metabolism of lyso-PLs. Therefore, analysis of lyso-PLs in human body fluids and tissues is not only an analytical, but also a biological issue. A good understanding of the biology of these lipids is essential for pathophysiologically relevant analyses of lyso-PLs. We will focus our discussion on: (1) a comparison of the electrospray ionization mass spectrometry (ESI-MS)-based method with other methods of lyso-PL analysis; (2) the influences of sample preparation on the analyses results; and (3) the issues related to sensitivity and reproducibility of the analyses, which are extremely important for biomarker identification and potential clinical use. The chapter includes discussions on the methods for qualitative and quantitative analysis of lyso-PLs in human body fluids (plasma, ascitic fluid, and urine) and in cells or cell culture media, as well as in animal tissues. The important factors that significantly affect the assays are emphasized. As future perspectives, we discuss the importance of further increasing the sensitivity and accuracy of the lyso-PL assays, as well as potential methods to establish high-throughput assays for lyso-PLs.

UR - http://www.scopus.com/inward/record.url?scp=74449087216&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74449087216&partnerID=8YFLogxK

U2 - 10.1201/9781420027655

DO - 10.1201/9781420027655

M3 - Chapter

SN - 9781574444674

SP - 125

EP - 145

BT - Functional Lipidomics

PB - CRC Press

ER -