Analysis of molecular marker expression reveals neuronal homology in distantly related arthropods

Molly Scheel, Nipam H. Patel

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Morphological studies suggest that insects and crustaceans of the Class Malacostraca (such as crayfish) share a set of homologous neurons. However, expression of molecular markers in these neurons has not been investigated, and the homology of insect and malacostracan neuroblasts, the neural stem cells that produce these neurons, has been questioned. Furthermore, it is not known whether crustaceans of the Class Branchiopoda (such as brine shrimp) or arthropods of the Order Collembola (springtails) possess neurons that are homologous to those of other arthropods. Assaying expression of molecular markers in the developing nervous systems of various arthropods could resolve some of these issues. Here, we examine expression of Even-skipped and Engrailed, two transcription factors that serve as insect embryonic CNS markers, across a number of arthropod species. This molecular analysis allows us to verify the homology of previously identified malacostracan neurons and to identify additional homologous neurons in malacostracans, collembolans and branchiopods. Engrailed expression in the neural stem cells of a number of crustaceans was also found to he conserved. We conclude that despite their distant phylogenetic relationships and divergent mechanisms of neurogenesis, insects, malacostracans, branchiopods and collembolans share many common CNS components.

Original languageEnglish (US)
Pages (from-to)2327-2334
Number of pages8
JournalDevelopment (Cambridge)
Volume126
Issue number11
StatePublished - 1999
Externally publishedYes

Fingerprint

Arthropods
Neurons
Insects
Neural Stem Cells
Artemia
Astacoidea
Neurogenesis
Nervous System
Transcription Factors
Cell Count

Keywords

  • Arthropod
  • Engrailed
  • Even-skipped
  • Homology
  • Neuroblast
  • Neurogenesis
  • Neuron

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Analysis of molecular marker expression reveals neuronal homology in distantly related arthropods. / Scheel, Molly; Patel, Nipam H.

In: Development (Cambridge), Vol. 126, No. 11, 1999, p. 2327-2334.

Research output: Contribution to journalArticle

@article{65fcd2832aa2477585bd5abb887b227e,
title = "Analysis of molecular marker expression reveals neuronal homology in distantly related arthropods",
abstract = "Morphological studies suggest that insects and crustaceans of the Class Malacostraca (such as crayfish) share a set of homologous neurons. However, expression of molecular markers in these neurons has not been investigated, and the homology of insect and malacostracan neuroblasts, the neural stem cells that produce these neurons, has been questioned. Furthermore, it is not known whether crustaceans of the Class Branchiopoda (such as brine shrimp) or arthropods of the Order Collembola (springtails) possess neurons that are homologous to those of other arthropods. Assaying expression of molecular markers in the developing nervous systems of various arthropods could resolve some of these issues. Here, we examine expression of Even-skipped and Engrailed, two transcription factors that serve as insect embryonic CNS markers, across a number of arthropod species. This molecular analysis allows us to verify the homology of previously identified malacostracan neurons and to identify additional homologous neurons in malacostracans, collembolans and branchiopods. Engrailed expression in the neural stem cells of a number of crustaceans was also found to he conserved. We conclude that despite their distant phylogenetic relationships and divergent mechanisms of neurogenesis, insects, malacostracans, branchiopods and collembolans share many common CNS components.",
keywords = "Arthropod, Engrailed, Even-skipped, Homology, Neuroblast, Neurogenesis, Neuron",
author = "Molly Scheel and Patel, {Nipam H.}",
year = "1999",
language = "English (US)",
volume = "126",
pages = "2327--2334",
journal = "Development",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "11",

}

TY - JOUR

T1 - Analysis of molecular marker expression reveals neuronal homology in distantly related arthropods

AU - Scheel, Molly

AU - Patel, Nipam H.

PY - 1999

Y1 - 1999

N2 - Morphological studies suggest that insects and crustaceans of the Class Malacostraca (such as crayfish) share a set of homologous neurons. However, expression of molecular markers in these neurons has not been investigated, and the homology of insect and malacostracan neuroblasts, the neural stem cells that produce these neurons, has been questioned. Furthermore, it is not known whether crustaceans of the Class Branchiopoda (such as brine shrimp) or arthropods of the Order Collembola (springtails) possess neurons that are homologous to those of other arthropods. Assaying expression of molecular markers in the developing nervous systems of various arthropods could resolve some of these issues. Here, we examine expression of Even-skipped and Engrailed, two transcription factors that serve as insect embryonic CNS markers, across a number of arthropod species. This molecular analysis allows us to verify the homology of previously identified malacostracan neurons and to identify additional homologous neurons in malacostracans, collembolans and branchiopods. Engrailed expression in the neural stem cells of a number of crustaceans was also found to he conserved. We conclude that despite their distant phylogenetic relationships and divergent mechanisms of neurogenesis, insects, malacostracans, branchiopods and collembolans share many common CNS components.

AB - Morphological studies suggest that insects and crustaceans of the Class Malacostraca (such as crayfish) share a set of homologous neurons. However, expression of molecular markers in these neurons has not been investigated, and the homology of insect and malacostracan neuroblasts, the neural stem cells that produce these neurons, has been questioned. Furthermore, it is not known whether crustaceans of the Class Branchiopoda (such as brine shrimp) or arthropods of the Order Collembola (springtails) possess neurons that are homologous to those of other arthropods. Assaying expression of molecular markers in the developing nervous systems of various arthropods could resolve some of these issues. Here, we examine expression of Even-skipped and Engrailed, two transcription factors that serve as insect embryonic CNS markers, across a number of arthropod species. This molecular analysis allows us to verify the homology of previously identified malacostracan neurons and to identify additional homologous neurons in malacostracans, collembolans and branchiopods. Engrailed expression in the neural stem cells of a number of crustaceans was also found to he conserved. We conclude that despite their distant phylogenetic relationships and divergent mechanisms of neurogenesis, insects, malacostracans, branchiopods and collembolans share many common CNS components.

KW - Arthropod

KW - Engrailed

KW - Even-skipped

KW - Homology

KW - Neuroblast

KW - Neurogenesis

KW - Neuron

UR - http://www.scopus.com/inward/record.url?scp=0033054435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033054435&partnerID=8YFLogxK

M3 - Article

C2 - 10225992

AN - SCOPUS:0033054435

VL - 126

SP - 2327

EP - 2334

JO - Development

JF - Development

SN - 0950-1991

IS - 11

ER -