Analysis of rat testicular protein expression following 91-day exposure to JP-8 jet fuel vapor

Frank Witzmann, Andrew Bobb, G. Bruce Briggs, Heather N. Coppage, Rex A. Hess, Junyu Li, Nathan M. Pedrick, Glenn D. Ritchie, John Rossi, Kenneth R. Still

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We analyzed protein expression in preparations from whole testis in adult male Sprague-Dawley rats exposed for 6 h/d for 91 consecutive days to jet propulsion fuel-8 (JP-8) in the vapor phase (0, 250, 500, or 1000 mg/m3 ± 10%), simulating a range of possible human occupational exposures. Whole body inhalation exposures were carefully controlled to eliminate aerosol phase, and subjects were sacrificed within 48 h postexposure. Organ fractions were solubilized and separated via large-scale, high resolution two-dimensional electrophoresis, and gel patterns scanned, digitized and processed for statistical analysis. Seventy-six different testis proteins were significantly increased or decreased in abundance in vapor-exposed groups, compared to controls, and dose-response profiles were often nonlinear. A number of the proteins were identified by peptide mass fingerprinting and related to histopathological or physiological deficits shown in previously published studies to occur with repeated exposure to hydrocarbon fuels or solvents. These results demonstrate a significant effect of JP-8 exposure on protein expression, particularly in protein expression in the rodent testis, and suggest that a 91 d exposure to jet fuel vapor induces changes of equal or greater magnitude to those reported previously for shorter duration JP-8 aerosol exposures.

Original languageEnglish
Pages (from-to)1016-1027
Number of pages12
JournalProteomics
Volume3
Issue number6
DOIs
StatePublished - Jun 1 2003

Fingerprint

Jet fuel
Propulsion
Rats
Vapors
Testis
Proteins
Aerosols
Inhalation Exposure
Peptide Mapping
Electrophoresis, Gel, Two-Dimensional
Occupational Exposure
Hydrocarbons
Electrophoresis
Sprague Dawley Rats
Rodentia
Statistical methods
Gels
Peptides

Keywords

  • Jet fuel
  • Rat
  • Testis
  • Toxicity
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Witzmann, F., Bobb, A., Briggs, G. B., Coppage, H. N., Hess, R. A., Li, J., ... Still, K. R. (2003). Analysis of rat testicular protein expression following 91-day exposure to JP-8 jet fuel vapor. Proteomics, 3(6), 1016-1027. https://doi.org/10.1002/pmic.200300385

Analysis of rat testicular protein expression following 91-day exposure to JP-8 jet fuel vapor. / Witzmann, Frank; Bobb, Andrew; Briggs, G. Bruce; Coppage, Heather N.; Hess, Rex A.; Li, Junyu; Pedrick, Nathan M.; Ritchie, Glenn D.; Rossi, John; Still, Kenneth R.

In: Proteomics, Vol. 3, No. 6, 01.06.2003, p. 1016-1027.

Research output: Contribution to journalArticle

Witzmann, F, Bobb, A, Briggs, GB, Coppage, HN, Hess, RA, Li, J, Pedrick, NM, Ritchie, GD, Rossi, J & Still, KR 2003, 'Analysis of rat testicular protein expression following 91-day exposure to JP-8 jet fuel vapor', Proteomics, vol. 3, no. 6, pp. 1016-1027. https://doi.org/10.1002/pmic.200300385
Witzmann, Frank ; Bobb, Andrew ; Briggs, G. Bruce ; Coppage, Heather N. ; Hess, Rex A. ; Li, Junyu ; Pedrick, Nathan M. ; Ritchie, Glenn D. ; Rossi, John ; Still, Kenneth R. / Analysis of rat testicular protein expression following 91-day exposure to JP-8 jet fuel vapor. In: Proteomics. 2003 ; Vol. 3, No. 6. pp. 1016-1027.
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