Analysis of sex chromosome distribution in the gonadal tissue of a 45,X/47,XYY mosaic by fluorescence in situ hybridization

Susanne Ragg, M. Harle, H. Scherthan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

45,X147,XYY mosaicism is a rare condition with scarce information on the tissuespecific distribution of the different cell lines. Fluorescence in situ hybridization with repetitive, chromosome-specific DNA probes was applied to gain insight into the tissue specific distribution of the two cell lines in biopsies of the streak gonad and dysgenetic testis of a 9-year-old individual exhibiting 45,X/ 47,XYY mosaicism. The distribution of the 45,X/47,XYY cells within different tissues was found to be nonrandom. The coelomic epithelium, the vascular endothelium, and the prepuberal germ cells exhibited predominantly a 4?Jm karyotype. In contrast, Sertoli cells exhibited both karyotypes, and the remaining tissue was predominantly composed of 45,X cells. The contribution of the two cell. lines to gonadal development is discussed.

Original languageEnglish (US)
Pages (from-to)295-298
Number of pages4
JournalModern Pathology
Volume8
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Sex Distribution
Sex Chromosomes
Fluorescence In Situ Hybridization
Mosaicism
Karyotype
Cell Line
Sertoli Cells
DNA Probes
Vascular Endothelium
Gonads
Tissue Distribution
Germ Cells
Testis
Epithelium
Chromosomes
Biopsy

Keywords

  • Fluorescence in situ hybridization
  • Interphase cytogenetics
  • Mixed gonadal dysgenesis
  • Mosaic
  • Paraffin
  • Sections

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Analysis of sex chromosome distribution in the gonadal tissue of a 45,X/47,XYY mosaic by fluorescence in situ hybridization. / Ragg, Susanne; Harle, M.; Scherthan, H.

In: Modern Pathology, Vol. 8, No. 3, 1995, p. 295-298.

Research output: Contribution to journalArticle

@article{61cb1d2877e044b8ae055b2221c0e46d,
title = "Analysis of sex chromosome distribution in the gonadal tissue of a 45,X/47,XYY mosaic by fluorescence in situ hybridization",
abstract = "45,X147,XYY mosaicism is a rare condition with scarce information on the tissuespecific distribution of the different cell lines. Fluorescence in situ hybridization with repetitive, chromosome-specific DNA probes was applied to gain insight into the tissue specific distribution of the two cell lines in biopsies of the streak gonad and dysgenetic testis of a 9-year-old individual exhibiting 45,X/ 47,XYY mosaicism. The distribution of the 45,X/47,XYY cells within different tissues was found to be nonrandom. The coelomic epithelium, the vascular endothelium, and the prepuberal germ cells exhibited predominantly a 4?Jm karyotype. In contrast, Sertoli cells exhibited both karyotypes, and the remaining tissue was predominantly composed of 45,X cells. The contribution of the two cell. lines to gonadal development is discussed.",
keywords = "Fluorescence in situ hybridization, Interphase cytogenetics, Mixed gonadal dysgenesis, Mosaic, Paraffin, Sections",
author = "Susanne Ragg and M. Harle and H. Scherthan",
year = "1995",
language = "English (US)",
volume = "8",
pages = "295--298",
journal = "Modern Pathology",
issn = "0893-3952",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Analysis of sex chromosome distribution in the gonadal tissue of a 45,X/47,XYY mosaic by fluorescence in situ hybridization

AU - Ragg, Susanne

AU - Harle, M.

AU - Scherthan, H.

PY - 1995

Y1 - 1995

N2 - 45,X147,XYY mosaicism is a rare condition with scarce information on the tissuespecific distribution of the different cell lines. Fluorescence in situ hybridization with repetitive, chromosome-specific DNA probes was applied to gain insight into the tissue specific distribution of the two cell lines in biopsies of the streak gonad and dysgenetic testis of a 9-year-old individual exhibiting 45,X/ 47,XYY mosaicism. The distribution of the 45,X/47,XYY cells within different tissues was found to be nonrandom. The coelomic epithelium, the vascular endothelium, and the prepuberal germ cells exhibited predominantly a 4?Jm karyotype. In contrast, Sertoli cells exhibited both karyotypes, and the remaining tissue was predominantly composed of 45,X cells. The contribution of the two cell. lines to gonadal development is discussed.

AB - 45,X147,XYY mosaicism is a rare condition with scarce information on the tissuespecific distribution of the different cell lines. Fluorescence in situ hybridization with repetitive, chromosome-specific DNA probes was applied to gain insight into the tissue specific distribution of the two cell lines in biopsies of the streak gonad and dysgenetic testis of a 9-year-old individual exhibiting 45,X/ 47,XYY mosaicism. The distribution of the 45,X/47,XYY cells within different tissues was found to be nonrandom. The coelomic epithelium, the vascular endothelium, and the prepuberal germ cells exhibited predominantly a 4?Jm karyotype. In contrast, Sertoli cells exhibited both karyotypes, and the remaining tissue was predominantly composed of 45,X cells. The contribution of the two cell. lines to gonadal development is discussed.

KW - Fluorescence in situ hybridization

KW - Interphase cytogenetics

KW - Mixed gonadal dysgenesis

KW - Mosaic

KW - Paraffin

KW - Sections

UR - http://www.scopus.com/inward/record.url?scp=0028969696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028969696&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 295

EP - 298

JO - Modern Pathology

JF - Modern Pathology

SN - 0893-3952

IS - 3

ER -