Analysis of T Cell Receptors Specific for Recognition of Class IB Antigens

Linda C. Lowen, Carla Aldrich, James Forman

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

T cells that recognize a peptide presented by a self-class IA molecule generally use a restricted repertoire of Vβ and Vα receptors. In contrast, alloreactive T cells, which recognize alloantigens that present a wide array of peptides, use a diverse repertoire, particularly in the CDR3 loop. Because the T cell repertoire directed against class IB alloantigens is not known, we examined V-D-J sequences in Vβ chains specific for Qa-1 and similar sequences in both Vβ and Vα chains specific for Qa-2. We observed that 14 Qa-1-specific clones use a limited number of Vβ segments and 8 of 14 express Vβ8.2 and have a conservation of charged residues in the CDR3 loop, particularly between residues 99 and 101. Thirteen of the 14 clones rearrange to the second Jβ cluster and use within this cluster is restricted. Alloreactive anti-Qa-1 T cells can be assigned into three different specificity groups based on a Qa-1 modifying gene, Qdm, as well as Qa-1 epitope expression on Tap-2-deficient RMA-S cells. Receptors from members of each specificity group are more similar in their CDR3 loop to each other than members of the other groups. These data lend support to the Qa-1 class IB Ag presenting a limited number of peptides to T cells or in some manner limiting the development of a diverse αβ T cell repertoire. The α- and β-chains from nine alloreactive anti-Qa-2 clones were analyzed. Vβ use was limited to use of Vβ7 or a member of the Vβ8 family. Rearrangements were solely to the second Jβ cluster. The use of Vα and Jα segments were diverse. Although conserved residues or motifs were observed in the CDR3 regions of both the β- and α-chains, the extent of conservation was less than that for anti-Qa-1 receptors. Anti-Qa-2 T cells can be divided into two specificities, Q6 and Q7. No common features were apparent between these groups.

Original languageEnglish (US)
Pages (from-to)6155-6165
Number of pages11
JournalJournal of Immunology
Volume151
Issue number11
StatePublished - Dec 1 1993
Externally publishedYes

Fingerprint

T-Cell Antigen Receptor
T-Lymphocytes
Antigens
Isoantigens
Clone Cells
Peptide T
Peptides
Epitopes
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Analysis of T Cell Receptors Specific for Recognition of Class IB Antigens. / Lowen, Linda C.; Aldrich, Carla; Forman, James.

In: Journal of Immunology, Vol. 151, No. 11, 01.12.1993, p. 6155-6165.

Research output: Contribution to journalArticle

Lowen, Linda C. ; Aldrich, Carla ; Forman, James. / Analysis of T Cell Receptors Specific for Recognition of Class IB Antigens. In: Journal of Immunology. 1993 ; Vol. 151, No. 11. pp. 6155-6165.
@article{fdefa25580e642a196900b8e26d6166b,
title = "Analysis of T Cell Receptors Specific for Recognition of Class IB Antigens",
abstract = "T cells that recognize a peptide presented by a self-class IA molecule generally use a restricted repertoire of Vβ and Vα receptors. In contrast, alloreactive T cells, which recognize alloantigens that present a wide array of peptides, use a diverse repertoire, particularly in the CDR3 loop. Because the T cell repertoire directed against class IB alloantigens is not known, we examined V-D-J sequences in Vβ chains specific for Qa-1 and similar sequences in both Vβ and Vα chains specific for Qa-2. We observed that 14 Qa-1-specific clones use a limited number of Vβ segments and 8 of 14 express Vβ8.2 and have a conservation of charged residues in the CDR3 loop, particularly between residues 99 and 101. Thirteen of the 14 clones rearrange to the second Jβ cluster and use within this cluster is restricted. Alloreactive anti-Qa-1 T cells can be assigned into three different specificity groups based on a Qa-1 modifying gene, Qdm, as well as Qa-1 epitope expression on Tap-2-deficient RMA-S cells. Receptors from members of each specificity group are more similar in their CDR3 loop to each other than members of the other groups. These data lend support to the Qa-1 class IB Ag presenting a limited number of peptides to T cells or in some manner limiting the development of a diverse αβ T cell repertoire. The α- and β-chains from nine alloreactive anti-Qa-2 clones were analyzed. Vβ use was limited to use of Vβ7 or a member of the Vβ8 family. Rearrangements were solely to the second Jβ cluster. The use of Vα and Jα segments were diverse. Although conserved residues or motifs were observed in the CDR3 regions of both the β- and α-chains, the extent of conservation was less than that for anti-Qa-1 receptors. Anti-Qa-2 T cells can be divided into two specificities, Q6 and Q7. No common features were apparent between these groups.",
author = "Lowen, {Linda C.} and Carla Aldrich and James Forman",
year = "1993",
month = "12",
day = "1",
language = "English (US)",
volume = "151",
pages = "6155--6165",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "11",

}

TY - JOUR

T1 - Analysis of T Cell Receptors Specific for Recognition of Class IB Antigens

AU - Lowen, Linda C.

AU - Aldrich, Carla

AU - Forman, James

PY - 1993/12/1

Y1 - 1993/12/1

N2 - T cells that recognize a peptide presented by a self-class IA molecule generally use a restricted repertoire of Vβ and Vα receptors. In contrast, alloreactive T cells, which recognize alloantigens that present a wide array of peptides, use a diverse repertoire, particularly in the CDR3 loop. Because the T cell repertoire directed against class IB alloantigens is not known, we examined V-D-J sequences in Vβ chains specific for Qa-1 and similar sequences in both Vβ and Vα chains specific for Qa-2. We observed that 14 Qa-1-specific clones use a limited number of Vβ segments and 8 of 14 express Vβ8.2 and have a conservation of charged residues in the CDR3 loop, particularly between residues 99 and 101. Thirteen of the 14 clones rearrange to the second Jβ cluster and use within this cluster is restricted. Alloreactive anti-Qa-1 T cells can be assigned into three different specificity groups based on a Qa-1 modifying gene, Qdm, as well as Qa-1 epitope expression on Tap-2-deficient RMA-S cells. Receptors from members of each specificity group are more similar in their CDR3 loop to each other than members of the other groups. These data lend support to the Qa-1 class IB Ag presenting a limited number of peptides to T cells or in some manner limiting the development of a diverse αβ T cell repertoire. The α- and β-chains from nine alloreactive anti-Qa-2 clones were analyzed. Vβ use was limited to use of Vβ7 or a member of the Vβ8 family. Rearrangements were solely to the second Jβ cluster. The use of Vα and Jα segments were diverse. Although conserved residues or motifs were observed in the CDR3 regions of both the β- and α-chains, the extent of conservation was less than that for anti-Qa-1 receptors. Anti-Qa-2 T cells can be divided into two specificities, Q6 and Q7. No common features were apparent between these groups.

AB - T cells that recognize a peptide presented by a self-class IA molecule generally use a restricted repertoire of Vβ and Vα receptors. In contrast, alloreactive T cells, which recognize alloantigens that present a wide array of peptides, use a diverse repertoire, particularly in the CDR3 loop. Because the T cell repertoire directed against class IB alloantigens is not known, we examined V-D-J sequences in Vβ chains specific for Qa-1 and similar sequences in both Vβ and Vα chains specific for Qa-2. We observed that 14 Qa-1-specific clones use a limited number of Vβ segments and 8 of 14 express Vβ8.2 and have a conservation of charged residues in the CDR3 loop, particularly between residues 99 and 101. Thirteen of the 14 clones rearrange to the second Jβ cluster and use within this cluster is restricted. Alloreactive anti-Qa-1 T cells can be assigned into three different specificity groups based on a Qa-1 modifying gene, Qdm, as well as Qa-1 epitope expression on Tap-2-deficient RMA-S cells. Receptors from members of each specificity group are more similar in their CDR3 loop to each other than members of the other groups. These data lend support to the Qa-1 class IB Ag presenting a limited number of peptides to T cells or in some manner limiting the development of a diverse αβ T cell repertoire. The α- and β-chains from nine alloreactive anti-Qa-2 clones were analyzed. Vβ use was limited to use of Vβ7 or a member of the Vβ8 family. Rearrangements were solely to the second Jβ cluster. The use of Vα and Jα segments were diverse. Although conserved residues or motifs were observed in the CDR3 regions of both the β- and α-chains, the extent of conservation was less than that for anti-Qa-1 receptors. Anti-Qa-2 T cells can be divided into two specificities, Q6 and Q7. No common features were apparent between these groups.

UR - http://www.scopus.com/inward/record.url?scp=0027377487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027377487&partnerID=8YFLogxK

M3 - Article

C2 - 8245458

AN - SCOPUS:0027377487

VL - 151

SP - 6155

EP - 6165

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 11

ER -