Analysis of the antifungal activity of ketoconazole, zinc pyrithione, and ciclopirox olamine against Pityrosporum ovale. A diffusion assay for cultures in solid media

Luis Squiquera, Lilian Plotkin, Irina Mathov, Ricardo Galimberti, Juliana Leoni

Research output: Contribution to journalArticle

5 Scopus citations


Background. Recently dandruff has been associated with a local increment in the number of Pityrosporum yeasts. Due to this fact, several anti-dandruff shampoos containing antifungals have been marketed. Objective. We studied the sensitivity of Pityrosporum ovale (PO) to three different groups of antimycotics (ketoconazole, Zn pyrithione, and ciclopirox olamine). Methods/Results. The drugs were tested by an inhibition assay in solid medium. Ketoconazole proved to be the most effective drug in inhibiting PO growth in a dose-dependent fashion. The results of our evaluation revealed that the inhibitory effects of the drugs were ketoconazole > Zn pyrithione > ciclopirox olamine. Conclusion. We postulate that the diffusion assay is a reliable medium for evaluating the effectiveness of antifungals contained in anti-dandruff shampoos.

Original languageEnglish (US)
Pages (from-to)26-29
Number of pages4
JournalJournal of the European Academy of Dermatology and Venereology
Issue number1
StatePublished - Jan 1 1996



  • Antifungals
  • Ketoconazole
  • Octopirox
  • Pityrosporum ovale
  • Zinc pyrithione

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

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