Analysis of the cytotoxic activity of carboplatin and gemcitabine combination

Sisi Wang, Hongyong Zhang, Liang Cheng, Christopher Evans, Chong Xian Pan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aim: To determine if the drug doses and administration schedules of carboplatin and gemcitabine combination affect antitumor effects. Materials and Methods: The inhibition of cell viability was measured by MTT assay. Median effect analysis was conducted to determine the cytotoxicity activity of carboplatin and gemcitabine combination. Cell cycle changes were analyzed by flow cytometry. Results: Synergism was observed when the bladder cancer cell line 5637 cells were treated with gemcitabine followed by carboplatin or concurrent carboplatin/gemcitabine. In contrast, moderate antagonism was observed when cells were treated with carboplatin followed by gemcitabine. Cell cycle analysis showed that the combined effect of these two drugs was cell cycle disturbance. Conclusion: Different doses and administration schedules affect the antitumor effect of carboplatin/ gemcitabine combination that may have clinical significance in the treatment for bladder cancer.

Original languageEnglish
Pages (from-to)4573-4578
Number of pages6
JournalAnticancer Research
Volume30
Issue number11
StatePublished - Nov 2010

Fingerprint

gemcitabine
Carboplatin
Cell Cycle
Urinary Bladder Neoplasms
Drug Administration Schedule
Cell Survival
Appointments and Schedules
Flow Cytometry
Cell Line

Keywords

  • Bladder cancer
  • Carboplatin
  • Cell cycle
  • Chemotherapy
  • Gemcitabine
  • Synergism

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wang, S., Zhang, H., Cheng, L., Evans, C., & Pan, C. X. (2010). Analysis of the cytotoxic activity of carboplatin and gemcitabine combination. Anticancer Research, 30(11), 4573-4578.

Analysis of the cytotoxic activity of carboplatin and gemcitabine combination. / Wang, Sisi; Zhang, Hongyong; Cheng, Liang; Evans, Christopher; Pan, Chong Xian.

In: Anticancer Research, Vol. 30, No. 11, 11.2010, p. 4573-4578.

Research output: Contribution to journalArticle

Wang, S, Zhang, H, Cheng, L, Evans, C & Pan, CX 2010, 'Analysis of the cytotoxic activity of carboplatin and gemcitabine combination', Anticancer Research, vol. 30, no. 11, pp. 4573-4578.
Wang, Sisi ; Zhang, Hongyong ; Cheng, Liang ; Evans, Christopher ; Pan, Chong Xian. / Analysis of the cytotoxic activity of carboplatin and gemcitabine combination. In: Anticancer Research. 2010 ; Vol. 30, No. 11. pp. 4573-4578.
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