Analysis of the Hand1 cell lineage reveals novel contributions to cardiovascular, neural crest, extra-embryonic, and lateral mesoderm derivatives

Ralston M. Barnes, Beth A. Firulli, Simon Conway, Joshua W. Vincentz, Anthony Firulli

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The basic Helix-Loop-Helix (bHLH) transcription factors Hand1 and Hand2 play critical roles in the development of multiple organ systems during embryogenesis. The dynamic expression patterns of these two factors within developing tissues obfuscate their respective unique and redundant organogenic functions. To define cell lineages potentially dependent upon Hand gene expression, we generated a mutant allele in which the coding region of Hand1 is replaced by Cre recombinase. Subsequent Cre-mediated activation of Β-galactosidase or eYFP reporter alleles enabled lineage trace analyses that clearly define the fate of Hand1-expressing cells. Hand1-driven Cre marks specific lineages within the extra embryonic tissues, placenta, sympathetic nervous system, limbs, jaw, and several cell types within the cardiovascular system. Comparisons between Hand1 expression and Hand1-lineage greatly refine our understanding of its dynamic spatial-temporal expression domains and raise the possibility of novel Hand1 functions in structures not thought to be Hand1-dependent. © 2010 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)3086-3097
Number of pages12
JournalDevelopmental Dynamics
Volume239
Issue number11
DOIs
StatePublished - Nov 2010

Fingerprint

Neural Crest
Cell Lineage
Mesoderm
Alleles
Galactosidases
Basic Helix-Loop-Helix Transcription Factors
Sympathetic Nervous System
Cardiovascular System
Jaw
Placenta
Embryonic Development
Extremities
Hand
Gene Expression
Cre recombinase

Keywords

  • BHLH
  • Cre recombinase
  • Hand factors
  • Lineage analysis

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Analysis of the Hand1 cell lineage reveals novel contributions to cardiovascular, neural crest, extra-embryonic, and lateral mesoderm derivatives. / Barnes, Ralston M.; Firulli, Beth A.; Conway, Simon; Vincentz, Joshua W.; Firulli, Anthony.

In: Developmental Dynamics, Vol. 239, No. 11, 11.2010, p. 3086-3097.

Research output: Contribution to journalArticle

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