Analysis of the mRNA coding for the chick vitamin D-induced calbindin and its regulation by 1,25-dihydroxyvitamin D3

Michael King, Anthony W. Norman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have used specific cloned cDNA probes generated from the mRNA coding for the vitamin D-induced 28,000-Da chick intestinal calcium binding protein (calbindin) to study the hormonal regulation of the expression of this mRNA by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. The calbindin-mRNA has been analyzed in chicken intestinal poly(A)+ mRNA samples as well as other chicken tissues by "Northern" blot analysis. There exists a predominant mRNA species of approximately 2000 nucleotides and two minor cross-hybridizing species that are nearly equivalent in proportion; their sizes are approximately 2600 and 3100 nucleotides. All three mRNA species are nonexistent in the chick intestine in the absence of vitamin D3 intake. However, all three mRNA species begin to accumulate at the same time in the chick intestine following the administration of the hormonally active metabolite of vitamin D3, 1,25-(OH)2D3. This response in the intestine is very similar to other steroid hormone-regulated gene products. All three mRNA species exist in the cell cytoplasm and are present on soluble polysome complexes, suggesting that all three are engaged in protein synthesis. Examination of other chick tissues (both vitamin D-deficient and -replete) reveals a close association between mRNA expression and previously observed calbindin expression. Each tissue is unique in the steady-state level of expression of the calbindin-mRNAs.

Original languageEnglish (US)
Pages (from-to)612-619
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume248
Issue number2
DOIs
StatePublished - Aug 1 1986
Externally publishedYes

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Calcium-Binding Proteins
Calcitriol
Vitamin D
Messenger RNA
Intestines
Cholecalciferol
Tissue
Chickens
Nucleotides
Steroid hormones
Polyribosomes
Metabolites
Northern Blotting
Cytoplasm
Complementary DNA
Genes
Steroids
Association reactions
Hormones

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Analysis of the mRNA coding for the chick vitamin D-induced calbindin and its regulation by 1,25-dihydroxyvitamin D3. / King, Michael; Norman, Anthony W.

In: Archives of Biochemistry and Biophysics, Vol. 248, No. 2, 01.08.1986, p. 612-619.

Research output: Contribution to journalArticle

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