Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting

Ching Pin Chang

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Formation of the cardiac outflow tract and great arteries involves complex morphogenetic processes, whose abnormities result in several clinically important diseases. Studies of these developmental processes are therefore important for understanding congenital vascular defects. However, the three-dimensional structure of arteries makes it challenging to analyze the pattern of vasculature using conventional histological approaches. Here we describe a vascular casting method to visualize the branching and connections of great arteries in developing embryos as well as in adult mice. This technique can be used to study the development of cardiac outflow tract, semilunar valves, and great arteries as demonstrated previously (Circ Res, 2008; Development 135: 3577-3586, 2008).

Original languageEnglish (US)
Title of host publicationCardiovascular Development
Subtitle of host publicationMethods and Protocols
EditorsXu Peng, Marc Antonyak
Pages21-28
Number of pages8
DOIs
StatePublished - Feb 1 2012

Publication series

NameMethods in Molecular Biology
Volume843
ISSN (Print)1064-3745

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Keywords

  • Bicuspid aortic valve
  • Cardiac outflow tract
  • Great arteries
  • Heart development
  • Heart valve
  • Overriding aorta
  • Persistent truncus arteriosus
  • Pulmonic stenosis
  • Tetralogy of Fallot
  • Vascular patterning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chang, C. P. (2012). Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting. In X. Peng, & M. Antonyak (Eds.), Cardiovascular Development: Methods and Protocols (pp. 21-28). (Methods in Molecular Biology; Vol. 843). https://doi.org/10.1007/978-1-61779-523-7_3