Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting

Ching-Pin Chang

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Formation of the cardiac outflow tract and great arteries involves complex morphogenetic processes, whose abnormities result in several clinically important diseases. Studies of these developmental processes are therefore important for understanding congenital vascular defects. However, the three-dimensional structure of arteries makes it challenging to analyze the pattern of vasculature using conventional histological approaches. Here we describe a vascular casting method to visualize the branching and connections of great arteries in developing embryos as well as in adult mice. This technique can be used to study the development of cardiac outflow tract, semilunar valves, and great arteries as demonstrated previously (Circ Res, 2008; Development 135: 3577-3586, 2008).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages21-28
Number of pages8
Volume843
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume843
ISSN (Print)10643745

Fingerprint

Blood Vessels
Angiography
Arteries
Embryonic Structures

Keywords

  • Bicuspid aortic valve
  • Cardiac outflow tract
  • Great arteries
  • Heart development
  • Heart valve
  • Overriding aorta
  • Persistent truncus arteriosus
  • Pulmonic stenosis
  • Tetralogy of Fallot
  • Vascular patterning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chang, C-P. (2012). Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting. In Methods in Molecular Biology (Vol. 843, pp. 21-28). (Methods in Molecular Biology; Vol. 843). https://doi.org/10.1007/978-1-61779-523-7_3

Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting. / Chang, Ching-Pin.

Methods in Molecular Biology. Vol. 843 2012. p. 21-28 (Methods in Molecular Biology; Vol. 843).

Research output: Chapter in Book/Report/Conference proceedingChapter

Chang, C-P 2012, Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting. in Methods in Molecular Biology. vol. 843, Methods in Molecular Biology, vol. 843, pp. 21-28. https://doi.org/10.1007/978-1-61779-523-7_3
Chang C-P. Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting. In Methods in Molecular Biology. Vol. 843. 2012. p. 21-28. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-523-7_3
Chang, Ching-Pin. / Analysis of the patterning of cardiac outflow tract and great arteries with angiography and vascular casting. Methods in Molecular Biology. Vol. 843 2012. pp. 21-28 (Methods in Molecular Biology).
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