Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation

Martin Fleischmann, Michael W. Clark, Wayne Forrester, Marvin Wickens, Takeharu Nishimoto, Markus Aebi

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Mutations in the PRP20 gene of yeast show a pleiotropic phenotype, in which both mRNA metabolism and nuclear structure are affected. srm1 mutants, defective in the same gene, influence the signal transduction pathway for the pheromone response. The yeast PRP20/SRM1 protein is highly homologous to the RCC1 protein of man, hamster and frog. In mammalian cells, this protein is a negative regulator for initiation of chromosome condensation. We report the analysis of two, independently isolated, recessive temperature-sensitive prp20 mutants. They have identical G to A transitions, leading to the alteration of a highly conserved glycine residue to glutamic acid. By immunofluorescence microscopy the PRP20 protein was localized in the nucleus. Expression of the RCC1 protein can complement the temperature-sensitive phenotype of prp20 mutants, demonstrating the functional similarity of the yeast and mammalian proteins.

Original languageEnglish (US)
Pages (from-to)417-423
Number of pages7
JournalMGG Molecular & General Genetics
Volume227
Issue number3
DOIs
StatePublished - Jul 1991
Externally publishedYes

Fingerprint

Chromosomes
Yeasts
Mutation
Proteins
Phenotype
Temperature
Fungal Proteins
Pheromones
Fluorescence Microscopy
Cricetinae
Anura
Glycine
Genes
Glutamic Acid
Signal Transduction
Complement System Proteins
Messenger RNA

Keywords

  • mRNA metabolism
  • Nucleus
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics

Cite this

Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation. / Fleischmann, Martin; Clark, Michael W.; Forrester, Wayne; Wickens, Marvin; Nishimoto, Takeharu; Aebi, Markus.

In: MGG Molecular & General Genetics, Vol. 227, No. 3, 07.1991, p. 417-423.

Research output: Contribution to journalArticle

Fleischmann, Martin ; Clark, Michael W. ; Forrester, Wayne ; Wickens, Marvin ; Nishimoto, Takeharu ; Aebi, Markus. / Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation. In: MGG Molecular & General Genetics. 1991 ; Vol. 227, No. 3. pp. 417-423.
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