Androgen regulation of gene expression in human meibomian gland and conjunctival epithelial cells

Payal Khandelwal, Shaohui Liu, David A. Sullivan

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Purpose: Androgens exert a significant influence on the structure, function and/or pathophysiology of the meibomian gland and conjunctiva. We sought to determine whether this hormone action involves the regulation of epithelial cell gene expression in these tissues. Methods: Immortalized human meibomian gland and conjunctival epithelial cells were treated with placebo or dihydrotestosterone (DHT) and processed for molecular biologic procedures. Gene expression was evaluated with BeadChips and data were analyzed with bioinformatic and statistical software. Results: Androgen treatment significantly influenced the expression of approximately 3,000 genes in immortalized human meibomian gland and conjunctival epithelial cells. The nature of DHT action on gene activity was predominantly cellspecific. Similarly, DHT exerted a significant, but primarily cell-specific, influence on many gene ontologies and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. These included groups of genes related, for example, to lipid dynamics, innate immunity, cell cycle, Janus kinase (JAK)-signal transducer and activator of transcription (stat) cascades, oxidative phosphorylation, the proteasome, and mammalian target of rapamycin (mTOR), Wnt, and peroxisome proliferator-activated receptor (PPAR) signaling. Conclusions: Our findings support our hypothesis that androgens regulate gene expression in human meibomian gland and conjunctival epithelial cells. Our ongoing studies are designed to determine whether many of these genes are translated and play a role in the health and well being of the eye.

Original languageEnglish (US)
Pages (from-to)1055-1067
Number of pages13
JournalMolecular Vision
Volume18
StatePublished - Apr 12 2012
Externally publishedYes

Fingerprint

Meibomian Glands
Gene Expression Regulation
Androgens
Epithelial Cells
Dihydrotestosterone
Genes
Gene Expression
Encyclopedias
Janus Kinases
Gene Ontology
Peroxisome Proliferator-Activated Receptors
Conjunctiva
Oxidative Phosphorylation
Proteasome Endopeptidase Complex
Sirolimus
Computational Biology
Transducers
Innate Immunity
Cell Cycle
Software

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Androgen regulation of gene expression in human meibomian gland and conjunctival epithelial cells. / Khandelwal, Payal; Liu, Shaohui; Sullivan, David A.

In: Molecular Vision, Vol. 18, 12.04.2012, p. 1055-1067.

Research output: Contribution to journalArticle

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