Angiogenesis and osteogenesis in an orthopedically expanded suture.

H. N. Chang, L. P. Garetto, R. H. Potter, Thomas Katona, C. H. Lee, W. E. Roberts

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The purpose of this study was to examine the angiogenic and the subsequent osteogenic responses during a 96-hour time-course after sutural expansion. Fifty rats were divided into: (1) a control group that received only angiogenic induction through injection of 5 ng/gm recombinant human endothelial cell growth factor (rhECGF); (2) an experimental group that received orthopedic expansion and rhECGF; (3) a sham group that received expansion and sodium chloride (NaCl) injection; and (4) a baseline group that received no expansion or injection. All rats were injected with 3H-thymidine (1.0 microCi/gm) 1 hour before death to label the DNA of S-phase cells. Demineralized sections (4 microm thick) were stained with hematoxylin and eosin. Angiogenesis and cell migration were analyzed with a previously established cell kinetics model. Analysis of variance was used to test the hypothesis that enhancement of angiogenesis stimulates reestablishment of osteogenic capability. Blood vessel number, area, and endothelial cell-labeled index significantly increased in experimental groups, but no difference was found between control and baseline groups. Labeled-pericyte index and activated pericyte numbers in the experimental group were also higher than in the sham groups. These results show that supplemental rhECGF enhances angiogenesis in expanded sutures but not in nonexpanded sutures. Data also suggest that pericytes are the source of osteoblasts in an orthopedically expanded suture.

Original languageEnglish (US)
Pages (from-to)382-390
Number of pages9
JournalAmerican Journal of Orthodontics and Dentofacial Orthopedics
Volume111
Issue number4
StatePublished - Apr 1997

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Endothelial Growth Factors
Osteogenesis
Pericytes
Sutures
Endothelial Cells
Injections
Control Groups
Hematoxylin
Eosine Yellowish-(YS)
Osteoblasts
S Phase
Sodium Chloride
Thymidine
Cell Movement
Orthopedics
Blood Vessels
Analysis of Variance
DNA

ASJC Scopus subject areas

  • Dentistry(all)
  • Surgery

Cite this

Chang, H. N., Garetto, L. P., Potter, R. H., Katona, T., Lee, C. H., & Roberts, W. E. (1997). Angiogenesis and osteogenesis in an orthopedically expanded suture. American Journal of Orthodontics and Dentofacial Orthopedics, 111(4), 382-390.

Angiogenesis and osteogenesis in an orthopedically expanded suture. / Chang, H. N.; Garetto, L. P.; Potter, R. H.; Katona, Thomas; Lee, C. H.; Roberts, W. E.

In: American Journal of Orthodontics and Dentofacial Orthopedics, Vol. 111, No. 4, 04.1997, p. 382-390.

Research output: Contribution to journalArticle

Chang, HN, Garetto, LP, Potter, RH, Katona, T, Lee, CH & Roberts, WE 1997, 'Angiogenesis and osteogenesis in an orthopedically expanded suture.', American Journal of Orthodontics and Dentofacial Orthopedics, vol. 111, no. 4, pp. 382-390.
Chang, H. N. ; Garetto, L. P. ; Potter, R. H. ; Katona, Thomas ; Lee, C. H. ; Roberts, W. E. / Angiogenesis and osteogenesis in an orthopedically expanded suture. In: American Journal of Orthodontics and Dentofacial Orthopedics. 1997 ; Vol. 111, No. 4. pp. 382-390.
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